Mi-RNA-888-5p Is Involved in S-Adenosylmethionine Antitumor Effects in Laryngeal Squamous Cancer Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. AdoMet Triggered Autophagy in LSCC
2.2. AdoMet Promoted ER-Stress and Related UPR in LSCC
2.3. AdoMet Induced ROS Production in LSCC
2.4. AdoMet Changed miRNA Expression Profile in LSCC
2.5. AdoMet and miR-888-5p Inhibitor Enhanced the Pro-Apoptotic Effect of AdoMet in LSCC
2.6. AdoMet and miR-888-5p Inhibitor Affect EMT in LSCC
2.7. AdoMet Regulates the Expression of MYCBP and CDH1 by the Downregulation of miR-888-5p
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Cultures and Treatments
4.3. LysoTracker-Red Staining
4.4. Determination of ROS by DCF-DA Assay
4.5. ER Tracker Blue-White DPX Staining
4.6. Cell Transfections
4.7. MiRNA Detection and Validation by qRT-PCR
4.8. Flow Cytometry Analysis of Apoptosis
4.9. RNA Isolation, Reverse Transcription, and qRT-PCR
4.10. Migration Process Evaluated by Scratch-Wound Assay
4.11. Protein Extraction and Western Blot Analysis
4.12. TCGAbiolinks Package
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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miRNAs | Fold-Changes (Log2) | |||
---|---|---|---|---|
JHU-SCC-011 | HNO210 | |||
Microarray | qRT-PCR | Microarray | qRT-PCR | |
hsa-miR-888-5p | −2.65 | −7.63 | Not performed | −1.98 |
hsa-miR-187 | 3.80 | 0.08 | Not performed | 0.01 |
hsa-miR-491-3p | 5.62 | 0.06 | Not performed | −0.12 |
hsa-miR-618 | −4.8 | −0.67 | Not performed | 0.23 |
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Pagano, M.; Mosca, L.; Vitiello, F.; Ilisso, C.P.; Coppola, A.; Borzacchiello, L.; Mele, L.; Caruso, F.P.; Ceccarelli, M.; Caraglia, M.; et al. Mi-RNA-888-5p Is Involved in S-Adenosylmethionine Antitumor Effects in Laryngeal Squamous Cancer Cells. Cancers 2020, 12, 3665. https://0-doi-org.brum.beds.ac.uk/10.3390/cancers12123665
Pagano M, Mosca L, Vitiello F, Ilisso CP, Coppola A, Borzacchiello L, Mele L, Caruso FP, Ceccarelli M, Caraglia M, et al. Mi-RNA-888-5p Is Involved in S-Adenosylmethionine Antitumor Effects in Laryngeal Squamous Cancer Cells. Cancers. 2020; 12(12):3665. https://0-doi-org.brum.beds.ac.uk/10.3390/cancers12123665
Chicago/Turabian StylePagano, Martina, Laura Mosca, Francesca Vitiello, Concetta Paola Ilisso, Alessandra Coppola, Luigi Borzacchiello, Luigi Mele, Francesca Pia Caruso, Michele Ceccarelli, Michele Caraglia, and et al. 2020. "Mi-RNA-888-5p Is Involved in S-Adenosylmethionine Antitumor Effects in Laryngeal Squamous Cancer Cells" Cancers 12, no. 12: 3665. https://0-doi-org.brum.beds.ac.uk/10.3390/cancers12123665