Chrysin-Induced Regression of Angiogenesis via an Induction of DNA Damage Response and Oxidative Stress in In Vitro and In Vivo Models of Melanoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. Drugs and Chemical Reagents
2.3. Viability Assays
2.4. Apoptosis Detection
2.5. Western Blot Analysis
2.6. Quantification of DNA Damage by Flow Cytometry
2.7. Immunofluorescence
2.8. Interaction Measurement with DNA G-Quadruplex/Duplex
2.9. Oxidative Stress Assays
2.10. RNA Extraction and Quantitative PCR Analysis
2.11. ELISA
2.12. Tumor Growth Analyses In Vivo
2.13. Densitometry and Statistical Significance
3. Results
3.1. Chrysin Prevents Melanoma Cell Proliferation
3.2. Chrysin Disrupts Cell Cycle Progression at the G2/M Phase and Induces Tetraploid Cells
3.3. Chrysin Induces DNA Damages, Leading to the Apoptosis Process
3.4. Chrysin Induces DNA Damage in Melanic Cell Lines
3.5. Chrysin Induces Moderate Binding to the G Quadruplex and Duplex Structures
3.6. Chrysin Decreases Key Actors of Angiogenesis Pathway
3.7. Chrysin Induces ROS Production
3.8. Chrysin Prevents Tumoral Growth and Angiogenesis In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sassi, A.; Fredon, M.; Cotte, A.K.; Fuselier, C.; Schneider, C.; Martiny, L.; Monchaud, D.; Chekir-Ghedira, L.; Aires, V.; Delmas, D. Chrysin-Induced Regression of Angiogenesis via an Induction of DNA Damage Response and Oxidative Stress in In Vitro and In Vivo Models of Melanoma. Cells 2023, 12, 1561. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121561
Sassi A, Fredon M, Cotte AK, Fuselier C, Schneider C, Martiny L, Monchaud D, Chekir-Ghedira L, Aires V, Delmas D. Chrysin-Induced Regression of Angiogenesis via an Induction of DNA Damage Response and Oxidative Stress in In Vitro and In Vivo Models of Melanoma. Cells. 2023; 12(12):1561. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121561
Chicago/Turabian StyleSassi, Aicha, Maxime Fredon, Alexia K. Cotte, Camille Fuselier, Christophe Schneider, Laurent Martiny, David Monchaud, Leila Chekir-Ghedira, Virginie Aires, and Dominique Delmas. 2023. "Chrysin-Induced Regression of Angiogenesis via an Induction of DNA Damage Response and Oxidative Stress in In Vitro and In Vivo Models of Melanoma" Cells 12, no. 12: 1561. https://0-doi-org.brum.beds.ac.uk/10.3390/cells12121561