Subcellular Localization of the TFF Peptides xP1 and xP4 in the Xenopus laevis Gastric/Esophageal Mucosa: Different Secretion Modes Reflecting Diverse Protective Functions
Abstract
:1. Introduction
2. Results
2.1. Localization of xP1 in the X. laevis Gastric Mucosa
2.2. Localization of xP4 in the X. laevis Esophagus
3. Discussion
3.1. Secretion of xP1 in Gastric Surface Mucous Cells: Concentration in Dense Core Regions of Secretory Granules
3.2. Secretion of xP4 in Esophageal Goblet Cells: Different Types of Secretory Granules and Goblet Cells
4. Materials and Methods
4.1. Preparation of Tissue Samples for Immunofluorescence and Immunoelectron Microscopy
4.2. Lectins and Antisera
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
DAPI | 4’,6-diamidino-2-phenylindole dihydrochloride |
TFF | Trefoil factor family |
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Goblet Cell | Granules | GSA-II/xP4 |
---|---|---|
Uniform-type (xP4-positive) | Type 1 | GSA-IIPOS/xP4POS |
Halo-type (xP4-negative) | Type 2 Type 3 | GSA-IIPOS/xP4NEG GSA-IINEG/xP4NEG |
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Schwarz, H.; Hoffmann, W. Subcellular Localization of the TFF Peptides xP1 and xP4 in the Xenopus laevis Gastric/Esophageal Mucosa: Different Secretion Modes Reflecting Diverse Protective Functions. Int. J. Mol. Sci. 2020, 21, 761. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21030761
Schwarz H, Hoffmann W. Subcellular Localization of the TFF Peptides xP1 and xP4 in the Xenopus laevis Gastric/Esophageal Mucosa: Different Secretion Modes Reflecting Diverse Protective Functions. International Journal of Molecular Sciences. 2020; 21(3):761. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21030761
Chicago/Turabian StyleSchwarz, Heinz, and Werner Hoffmann. 2020. "Subcellular Localization of the TFF Peptides xP1 and xP4 in the Xenopus laevis Gastric/Esophageal Mucosa: Different Secretion Modes Reflecting Diverse Protective Functions" International Journal of Molecular Sciences 21, no. 3: 761. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21030761