miR-142-3p Expression Is Predictive for Severe Traumatic Brain Injury (TBI) in Trauma Patients
Abstract
:1. Introduction
2. Results
2.1. Pilot Study 1: Analyzing miRNA Concentrations in Serum and Plasma Samples of Trauma Patients
2.2. Pilot Study 2: Identification of a Suitable Housekeeping miRNA in Trauma Patients
2.3. Demographic and Injury Characteristics
2.4. Detection Profile of miRNAs in Trauma Patients Stratified by Injury Pattern
2.5. Quantitative Analysis of miR-124-3p, miR-338-3p and miR-423-3p Expression in Trauma Patients
2.6. Relation and Predictive Power of miR-423-3p in Severity of Injury
2.7. miR-423-3p Pathway Analysis
3. Discussion
Limitations of the Study
4. Materials and Methods
Statistical Analysis
- (1).
- ∆CtTarget = CtSNORD95 – CtTarget;
- (2).
- ∆∆CtTarget = ∆Ct[V] – ∆CtTarget;
- (3).
- 2−∆∆Ct.
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
AIS | Abbreviated Injury Score |
ISS | Injury Severity Score |
NISS | New Injury Severity Score |
RISC II | Revised Injury Severity Classification Score II |
PT | Polytrauma |
(is)TBI | (isolated) Traumatic Brain Injury |
MOF | multiple organ failure |
BBB | Blood–brain barrier |
SNORD | small nuclear RNAs |
GCS | Glasgow Coma scale |
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PT (n = 8) | PT + TBI (n = 13) | isTBI (n = 12) | |
---|---|---|---|
Median (IQR) | Median (IQR) | Median (IQR) | |
Age (y) | 52 (42–66) | 48 (32–61) | 71 (55–77) |
ISS (pts) | 37 (24–43) | 34 (32–42) | 25 (21–25) |
NISS (pts) | 47 (31–50) | 48 (39–57) | 45 (37–62) |
RISC II (%) | 4 (2–11) | 39 (14–60) | 33 (17–83) |
AIShead (pts) | 0 | 5 (4–5) | 5 (4–5) |
AISthorax (pts) | 4 (3–4) | 3 (3–4) | 0 |
AISabdominal (pts) | 3 (2–4) | 0 (0–1) | 0 |
AISextremities (pts) | 4 (2–5) | 0 (0–3) | 0 |
GCS (pts) | 15 (11–15) | 3 (3–8) | 3 (3–12) |
miR-124-3p | miR-338-3p | miR-423-3p | |||||
---|---|---|---|---|---|---|---|
n | mean ± SD | n | mean ± SD | n | mean ± SD | ||
PT | Ct | 1 | 39.8 ± 0 | 8 | 32.4 ± 2.9 | ||
∆Ct | 13.6 ± 0 | 4.7 ± 1.2 | |||||
∆∆Ct | 13.6 ± 0 | 2.6 ± 1.2 | |||||
2−∆∆Ct | 0.0 | 0.2 ± 0.2 | |||||
PT + TBI | Ct | 7 | 37.0 ± 3.0 | 3 | 38.2 ± 1.3 | 13 | 31.3 ± 3.5 |
∆Ct | 11.8 ± 3.8 | 13.5 ± 1.9 | 3.9 ± 1.3 | ||||
∆∆Ct | 11.8 ± 3.8 | 13.5 ± 1.9 | 1.8 ± 1.3 | ||||
2−∆∆Ct | 0.0 | 0.0 | 0.4 ± 0.2 | ||||
isTBI | Ct | 4 | 34.7 ± 2.7 | 5 | 38.3 ± 1.6 | 12 | 30.7 ± 1.4 |
∆Ct | 7.5 ± 1.3 | 10.4 ± 2.0 | 2.4 ± 1.5 | ||||
∆∆Ct | 7.5 ± 1.3 | 10.4 ± 2.0 | 0.3 ± 1.5 | ||||
2−∆∆Ct | 0.0 | 0.0 | 1.5 ± 2.3 |
Target Rank | Target Score | Gene Symbol | Gene Description | Protein Function |
---|---|---|---|---|
1 | 96 | PABPC1 | poly(A) binding protein cytoplasmic 1 | Binds to RNA, translation initiation |
2 | 85 | PABPC3 | poly(A) binding protein cytoplasmic 3 | Binds to RNA, translation initiation |
3 | 83 | RAP2C | RAP2C, member of RAS oncogene family | small GTPases that act as molecular switches to regulate cellular proliferation, differentiation, and apoptosis |
4 | 78 | FRY | FRY microtubule binding protein | Cell morphogenesis |
5 | 77 | CBX7 | chromobox 7 | Controls the lifespan of several normal human cells |
6 | 77 | CREM | cAMP responsive element modulator | Transcription factor that binds to the cAMP responsive element |
7 | 76 | RAB14 | RAB14, member RAS oncogene family | Low-molecular mass GTPase, involved in intracellular membrane trafficking |
8 | 74 | FGFR2 | fibroblast growth factor receptor 2 | The extracellular portion of the protein interacts with fibroblast growth factors, ultimately influencing mitogenesis and differentiation. |
9 | 73 | BCORL1 | BCL6 corepressor like 1 | Can interact with several different class II histone deacetylases to repress transcription. |
10 | 70 | RAC1 | Rac family small GTPase 1 | GTPase which belongs to the RAS superfamily, appears to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization and the activation of protein kinases. |
11 | 70 | ESRRA | estrogen related receptor alpha | Nuclear receptor that is most closely related to the estrogen receptor, acts as a site-specific transcription factor |
12 | 70 | ITGA11 | integrin subunit alpha 11 | Encodes an alpha integrin. |
13 | 69 | LGALSL | galectin like | unknown |
14 | 68 | GYPC | glycophorin C (Gerbich blood group) | Plays an important role in regulating the mechanical stability of red cells. |
15 | 66 | PROZ | protein Z, vitamin K dependent plasma glycoprotein | The encoded protein plays a role in regulating blood coagulation |
16 | 65 | HS6ST2 | heparan sulfate 6-O-sulfotransferase 2 | Interacts with various ligands to influence cell growth, differentiation, adhesion and migration |
17 | 65 | ZNF135 | zinc finger protein 135 | unknown |
18 | 64 | DLL1 | delta like canonical Notch ligand 1 | Plays a role in mediating cell fate decisions during hematopoiesis |
19 | 62 | ZBTB46 | zinc finger and BTB domain containing 46 | unknown |
20 | 60 | CRK | CRK proto-oncogene, adaptor protein | Is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase |
21 | 59 | ACOX3 | acyl-CoA oxidase 3, pristanoyl | Is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. |
22 | 59 | TRDN | triadin | Integral membrane protein that contains a single transmembrane domain |
23 | 59 | DKK3 | dickkopf WNT signaling pathway inhibitor 3 | Involved in embryonic development through its interactions with the Wnt signaling pathway. It may function as a tumor suppressor gene. |
24 | 58 | KLHL29 | kelch like family member 29 | Binding interactions with other proteins, e.g., actins |
25 | 54 | SLC11A2 | solute carrier family 11 member 2 | Transports divalent metals and is involved in iron absorption. |
26 | 54 | ZNF16 | zinc finger protein 16 | Is involved in the differentiation of erythroid and megakaryocytic cells. |
27 | 51 | PLCH1 | phospholipase C eta 1 | cleaves PtdIns (4,5) P2 to generate second messengers IP3 and DAG |
28 | 50 | CALML3 | calmodulin like 3 | unknown |
29 | 50 | GIPC3 | GIPC PDZ domain containing family member 3 | required for postnatal maturation of the hair bundle and long-term survival of hair cells and spiral ganglion in the ear. |
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Schindler, C.R.; Woschek, M.; Vollrath, J.T.; Kontradowitz, K.; Lustenberger, T.; Störmann, P.; Marzi, I.; Henrich, D. miR-142-3p Expression Is Predictive for Severe Traumatic Brain Injury (TBI) in Trauma Patients. Int. J. Mol. Sci. 2020, 21, 5381. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155381
Schindler CR, Woschek M, Vollrath JT, Kontradowitz K, Lustenberger T, Störmann P, Marzi I, Henrich D. miR-142-3p Expression Is Predictive for Severe Traumatic Brain Injury (TBI) in Trauma Patients. International Journal of Molecular Sciences. 2020; 21(15):5381. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155381
Chicago/Turabian StyleSchindler, Cora Rebecca, Mathias Woschek, Jan Tilmann Vollrath, Kerstin Kontradowitz, Thomas Lustenberger, Philipp Störmann, Ingo Marzi, and Dirk Henrich. 2020. "miR-142-3p Expression Is Predictive for Severe Traumatic Brain Injury (TBI) in Trauma Patients" International Journal of Molecular Sciences 21, no. 15: 5381. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155381