Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas
Abstract
:1. Introduction
2. Results
2.1. hTECs Wound Healing Alters CLU Gene Expression in Human Corneal Epithelial Cells
2.2. CLU Gene Transcription Is Modulated by Both Positive and Negative Regulatory Elements
2.3. Analysis of the Transcription Factors Binding to the CLU Basal Promoter in HCECs
2.4. Members from the AP1 and Sp1 Families Bind to the CLU Basal Promoter in hCECs
2.5. Positioning of the Functional Sp1/Sp3 Binding Site in the CLU Promoter In Vitro
2.6. Influence of Cell Damage on the Binding of Sp1/Sp3 to the CLU Promoter in hCECs
3. Discussion
4. Materials and Methods
4.1. Cell Culture—Human Epithelial Cells (hCECs)
4.2. Human Tissue-Engineered Cornea (hTEC)
4.3. Gene Expression Profiling
4.4. Western Blot Analyses
4.5. Plasmid Constructs and Oligonucleotides
4.6. Transient Transfection and CAT (Chloramphenicol Acetyl Transferase) Assays
4.7. Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assays (EMSA)
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gross, C.; Le-Bel, G.; Desjardins, P.; Benhassine, M.; Germain, L.; Guérin, S.L. Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas. Int. J. Mol. Sci. 2021, 22, 12426. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212426
Gross C, Le-Bel G, Desjardins P, Benhassine M, Germain L, Guérin SL. Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas. International Journal of Molecular Sciences. 2021; 22(22):12426. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212426
Chicago/Turabian StyleGross, Christelle, Gaëtan Le-Bel, Pascale Desjardins, Manel Benhassine, Lucie Germain, and Sylvain L. Guérin. 2021. "Contribution of the Transcription Factors Sp1/Sp3 and AP-1 to Clusterin Gene Expression during Corneal Wound Healing of Tissue-Engineered Human Corneas" International Journal of Molecular Sciences 22, no. 22: 12426. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212426