PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos
Abstract
:1. Introduction
2. Results
2.1. Effects of PAHs and PCBs on Gene Expression by Real Time qPCR
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- Stress genes:
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- Genes involved in development/differentiation:
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- Genes involved in detoxification:
2.2. Network Analysis
3. Discussion
4. Materials and Methods
4.1. Experimental Conditions
4.2. RNA Extraction and cDNA Synthesis
4.3. Network Analysis
4.4. RT-qPCR Analysis
4.5. Gene Networks
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Names | Acronym | Accession Number | Primer | Sequence (5′→3′) | Amplicon Lenght (bp) |
---|---|---|---|---|---|
Calcium/calmodulin-dependent protein kinase type 1D | CM-K | PRJNA495004 | Pl_CM_F1 | GTTATCCTCCATTTTACGATGAG | 168 |
Pl_CM_R1 | GCAGATATACGTGTGAGGAAG | ||||
Camp-responsive element | CREB | PRJNA495004 | Pl_CREB_F2 | GTAACTAAAGCATCTGGGAGAC | 158 |
Pl_CREB_R2 | GGTTCAGATATTAGTGGATGC | ||||
Cholinesterase | ChE | SUB6701449 | Pl_ChE_F2 | CGAGATGGCGTATGTTTTGAG | 160 |
Pl_ChE_R2 | GACTATGTTCCCGCTGACTG | ||||
Citochrome P450 2UI isoform X2 | CYP-2UI | SUB6701449 | Pl_CYP-2UI_F1 | GCGCCTCTTCGTTCTATTCC | 174 |
Pl_CUP-2UI_R1 | CGGCATAGTAGTAGACTAGC | ||||
Epidermal growth factor | EGF | PRJNA495004 | Pl_EGF_F1 | CGGCGGTGTGTGTATCGATG | 189 |
Pl_EGF_R1 | CAGTAGCCATCCTAGTGTTCC | ||||
Frizzled7 | FZ-7 | PRJNA495004 | Pl_FZ_F1 | GATCGTGAGCGTAGCATATAC | 175 |
Pl_FZ_R1 | CATGGTCTTTTTGGGCACTA | ||||
Glutathione-S-transferase | GST | SUB6701449 | Pl_GST_F4 | GCCCGACTTACCTACTTTGC | 165 |
Pl_GST_R4 | CTTGCAGCTCATCACTGATGG | ||||
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | PRJNA495004 | Pl_GAPDH_F1 | GTACTACTTCTCATTCACCTTG | 213 |
Pl_GAPDH_R1 | CATAGCTCTGACACCGCCAC | ||||
heat shock protein 75 | hsp75 | PRJNA495004 | Pl_hsp75_F2 | GGACTGGTGGAACAACTATATC | 173 |
Pl_hsp75_R2 | CGATCACCACTCTCTGTCAC | ||||
heat shock protein 90 | hsp90 | SUB6701449 | Pl_hsp90_F1 | GGGTGTGGTAGATTCTGATG | 148 |
Pl_hsp90_R1 | GCTCTCCATGTATTCATCAG | ||||
Hedgehog | HH | PRJNA495004 | Pl_HH_F1 | GGTACATGAGGCACAAGCTAG | 193 |
Pl_HH_R1 | CCACTTCACATCACTTGACC | ||||
Janus kinase | JAK | XM_030985987 | Pl_JAK_F2 | CACCTTACCCATACTAGACAG | 192 |
Pl_JAK_R2 | CTTGCCAGAGCCTCCGCTGAC | ||||
Lefty | Lefty | SUB6701449 | Pl_Lefty_F2 | CAGTCCAGACATGGGTGGCAG | 182 |
Pl_Lefty_R2 | CATTTCGTCGACCACCTGCTG | ||||
maternal Vg1 | M-Vg1 | SUB6701449 | Pl_M-Vg1_F1 | GCACCTGCACCTAGAGACTC | 145 |
Pl_M-Vg1_R1 | GCATGACCTTTTCCGGCCTG | ||||
NADH dehydrogenase | NADH | SUB2817153 | Pl_NADH_F1 | GTCTCCGTCGGATAAATCAAAG | 194 |
Pl_NADH_R1 | CCGAAAAGGAAATAACGAAGC | ||||
Nemo-like kinase protein | NLK | AY442297 | Pl_NLK_F1 | CCTCTACCAGATTCTCAGAG | 192 |
Pl_NLK_R1 | GTGACACAGTACTACCGCGC | ||||
Notchless protein | NOTCHLESS | PRJNA495004 | Pl_Notchless_F1 | GGGAAGCTAAGGCATCAGAC | 145 |
Pl_Notchless_R1 | CGATCCTCTCAAGCACTTTAG | ||||
Patched | Ptc | SUB6701449 | Pl_Ptc_F1 | CGGTCGTCAGTATCATCATG | 135 |
Pl_Ptc_R1 | GCAACCACGACTCCGTAAGC | ||||
Phospholipase C | PLC | AJ012336 | Pl_PLC_F1 | GAGACATTCACAGTGCCCAC | 139 |
Pl_PLC_R1 | CTGACCGATACCAAGCTGTAC | ||||
PLAUF 3 RNA-binding protein AUF1 mRNA | PLAUF 3 | AY682309.1 | Pl_PLAUF3_F2 | GGAGGATACGGCGGTGGCGG | 182 |
Pl_PLAUF3_R2 | GTGTTGACTCCACAGGAGTG | ||||
Polyketide synthase | PKS | SUB6701449 | Pl_PKS_F1 | GCTTCCTCGACCAGTCTGTC | 142 |
Pl_PKS_R1 | CCTCCGAAGACAGTCATCTG | ||||
signal transducer and activator of transcription | STAT1 | PRJNA495004 | Pl_STAT_F1 | GTGTGTCAATCAGCCAGTGC | 196 |
Pl_STAT_R1 | GTACATCATGAGCTTACCATTTC | ||||
Smoothened | Smo | SUB6701449 | Pl_Smo_F1 | CACGATCCATTACGGCGTTG | 217 |
Pl_Smo_R1 | GCCCAACTCACACCCATGAC | ||||
sulfotransferase 1C2-like | SULT1 | SUB6701449 | Pl_SULT1_F2 | CAGGCACTCACTGGCTCATG | 140 |
Pl_SULT1_R2 | CTCTTCAGCTCTCGTCTTCG | ||||
Tumor necrosis factor alpha | TNF | SUB2817153 | Pl_TNF_F1 | CCTGATGTGTATGCCTCTATC | 144 |
Pl_TNF_R1 | CAAGATCCTCATGTCAGGAAG |
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Albarano, L.; Zupo, V.; Guida, M.; Libralato, G.; Caramiello, D.; Ruocco, N.; Costantini, M. PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos. Int. J. Mol. Sci. 2021, 22, 12498. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212498
Albarano L, Zupo V, Guida M, Libralato G, Caramiello D, Ruocco N, Costantini M. PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos. International Journal of Molecular Sciences. 2021; 22(22):12498. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212498
Chicago/Turabian StyleAlbarano, Luisa, Valerio Zupo, Marco Guida, Giovanni Libralato, Davide Caramiello, Nadia Ruocco, and Maria Costantini. 2021. "PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos" International Journal of Molecular Sciences 22, no. 22: 12498. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212498