Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice
Abstract
:1. Introduction
2. Results
2.1. Maternal Variables
2.2. Fetal Variables
2.3. Triglycerides
2.4. Placenta
3. Discussion
4. Materials and Methods
4.1. Mouse Model
4.2. Experimental Design
4.3. Dual-Energy X-ray Absorptiometry
4.4. Serum Measurements
4.5. Liver and Placenta Triglyceride Content
4.6. Placenta Glycogen Content
4.7. Quantitative Real-Time PCR
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Maternal Variables | Wt CD | APN Het CD | APN Ko CD | Wt HF/HS | APN Het HF/HS | APN Ko HF/HS | Two-Way ANOVA |
---|---|---|---|---|---|---|---|
n = 6 | n = 8 | n = 11 | n = 6 | n = 8 | n = 8 | ||
Body weight (g) | 40.9 ± 1.0 | 35.0 ± 0.9 ** | 41.2 ± 1.4 | 37.3 ± 1.9 | 38.3 ± 1.1 | 39.9 ± 0.7 | |
Blood glucose (mmol/L) | 5.58 ± 0.16 | 6.66 ± 0.52 | 6.77 ± 0.42 * | 6.26 ± 0.45 | 6.33 ± 0.20 | 5.49 ± 0.29 | |
Insulin (µg/L) | 1.29 ± 0.33 | 1.16 ± 0.28 | 1.37 ± 0.36 | 0.78 ±0.25 | 1.98 ± 0.40 | 0.93 ± 0.29 | |
Heart (mg) | 115 ± 3 | 113 ± 3 | 124 ± 4 | 131 ± 7 | 135 ± 4 | 150 ± 6 | a; F(1, 39) = 29.8, p < 0.001 b; F(2, 39) = 5.57, p = 0.007 |
Subcutaneous inguinal fat (g) | 0.62 ± 0.05 | 0.39 ± 0.03 ** | 0.52 ± 0.05 | 0.86 ± 0.18 | 0.72 ± 0.07 | 0.89 ± 0.10 | a; F(1, 38) = 19.9, p < 0.001 |
Mesenteric fat (g) | 0.38 ± 0.03 | 0.29 ± 0.02 | 0.32 ± 0.04 | 0.35 ± 0.08 | 0.32 ± 0.03 | 0.39 ± 0.07 | |
Brown adipose tissue (mg) | 62.6 ± 3.4 | 58.7 ± 4.6 | 72.3 ± 7.1 | 80.6 ± 8.7 | 69.4 ± 1.9 | 78.6 ± 6.6 | a; F(1, 39) = 5.73, p = 0.022 |
Liver (g) | 1.57 ± 0.06 | 1.58 ± 0.07 | 1.74 ± 0.05 | 2.17 ± 0.12 | 2.32 ± 0.13 | 2.43 ± 0.142 | a; F(1, 39) = 65.9, p < 0.001 |
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Mohan Shrestha, M.; Wermelin, S.; Stener-Victorin, E.; Wernstedt Asterholm, I.; Benrick, A. Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice. Int. J. Mol. Sci. 2022, 23, 4939. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23094939
Mohan Shrestha M, Wermelin S, Stener-Victorin E, Wernstedt Asterholm I, Benrick A. Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice. International Journal of Molecular Sciences. 2022; 23(9):4939. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23094939
Chicago/Turabian StyleMohan Shrestha, Man, Sanne Wermelin, Elisabet Stener-Victorin, Ingrid Wernstedt Asterholm, and Anna Benrick. 2022. "Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice" International Journal of Molecular Sciences 23, no. 9: 4939. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23094939