Y192 within the SH2 Domain of Lck Regulates TCR Signaling Downstream of PLC-γ1 and Thymic Selection
Abstract
:1. Introduction
2. Results
2.1. Defective Thymocyte Selection in LckY192E Mice
2.2. LckY192E Initiates TCR Signaling in Thymocytes
2.3. The TCR Is Uncoupled from the Activation of Pathways Downstream of PLC-γ1 in LckY192E Thymocytes
3. Discussion
4. Materials and Methods
4.1. Antibodies
4.2. Mice
4.3. Stimulation and Lysis of Cells
4.4. Immunoblotting
4.5. Flow Cytometry Measurements
4.6. Calcium Flux
4.7. Intracellular Staining
4.8. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kästle, M.; Merten, C.; Hartig, R.; Plaza-Sirvent, C.; Schmitz, I.; Bommhardt, U.; Schraven, B.; Simeoni, L. Y192 within the SH2 Domain of Lck Regulates TCR Signaling Downstream of PLC-γ1 and Thymic Selection. Int. J. Mol. Sci. 2022, 23, 7271. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23137271
Kästle M, Merten C, Hartig R, Plaza-Sirvent C, Schmitz I, Bommhardt U, Schraven B, Simeoni L. Y192 within the SH2 Domain of Lck Regulates TCR Signaling Downstream of PLC-γ1 and Thymic Selection. International Journal of Molecular Sciences. 2022; 23(13):7271. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23137271
Chicago/Turabian StyleKästle, Matthias, Camilla Merten, Roland Hartig, Carlos Plaza-Sirvent, Ingo Schmitz, Ursula Bommhardt, Burkhart Schraven, and Luca Simeoni. 2022. "Y192 within the SH2 Domain of Lck Regulates TCR Signaling Downstream of PLC-γ1 and Thymic Selection" International Journal of Molecular Sciences 23, no. 13: 7271. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23137271