Side-Chain Immune Oxysterols Induce Neuroinflammation by Activating Microglia
Abstract
:1. Introduction
2. Results
2.1. Increased Expression of IL-1β in Microglia following Treatment with 25OHChol, 27OHChol, Aβ, and LPS
2.2. Concentration and Time-Course Effects of 25OHChol and 27OHChol on IL-1β Expression
2.3. Involvement of Multiple Signaling Pathways in the Expression of IL-1β Induced by 25OHChol and 27OHChol
2.4. Upregulated Expression of MHC II on the Cell Surface following Stimulation with 25OHChol and 27OHChol
2.5. Enhanced Microglial Expression of IL-1β and MHC II in ApoE-Deficient Mice
2.6. Suppressive Effects of Cyclosporin A (CsA) and Chlorpromazine (CPZ) on the Expression of IL-1β and the Activation of Microglia Induced by 25OHChol and 27OHChol
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Reverse Transcriptase and Real-Time Polymerase Chain Reaction (RT and Real-Time PCR)
4.3. Western Blot Analysis
4.4. ELISA
4.5. Immunofluorescence of MHC II
4.6. Immunofluorescence Staining of Murine Brain Sections
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Son, Y.; Yeo, I.-J.; Hong, J.-T.; Eo, S.-K.; Lee, D.; Kim, K. Side-Chain Immune Oxysterols Induce Neuroinflammation by Activating Microglia. Int. J. Mol. Sci. 2023, 24, 15288. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242015288
Son Y, Yeo I-J, Hong J-T, Eo S-K, Lee D, Kim K. Side-Chain Immune Oxysterols Induce Neuroinflammation by Activating Microglia. International Journal of Molecular Sciences. 2023; 24(20):15288. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242015288
Chicago/Turabian StyleSon, Yonghae, In-Jun Yeo, Jin-Tae Hong, Seong-Kug Eo, Dongjun Lee, and Koanhoi Kim. 2023. "Side-Chain Immune Oxysterols Induce Neuroinflammation by Activating Microglia" International Journal of Molecular Sciences 24, no. 20: 15288. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242015288