Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.)
Abstract
:1. Introduction
2. Results
2.1. Melatonin Applications Significantly Alleviate Al-Induced Growth Inhibition in Alfalfa
2.2. Melatonin Could Reduce the Accumulation of Al under Al Stress in Alfalfa
2.3. Melatonin Could Alleviate Al-Induced Oxidative Damage in Root Cells
2.4. Melatonin Could Enhance the Activity of Antioxidant Enzymes
2.5. Treatments with Al and Melatonin Trigger Transcriptome Reprogramming in Alfalfa Roots
2.6. Gene Ontology (GO) Classification and KEGG Pathway Analysis
3. Discussion
3.1. Exogenous Melatonin Reduced Al Concentration in Both Alfalfa Roots and Shoots
3.2. Exogenous Melatonin Enhanced the Antioxidant Capacity in Alfalfa
3.3. Exogenous Melatonin Elevated C Metabolism under Al Stress in Alfalfa
4. Materials and Methods
4.1. Plant Culture and Measurements of Al Treatments
4.2. Anatomical Observation of Root Tip Cells
4.3. Morin Staining
4.4. Determination of Al Content
4.5. Determination of H2O2 Content
4.6. Analysis of Oxidative Damage
4.7. Determination of Antioxidant Enzyme Activity
4.8. RNA Extraction and Transcriptome Sequencing
4.9. Differential Expression Analysis
4.10. GO and KEGG Enrichment Analysis of DEGs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, C.; Cheng, H.; Wang, S.; Yu, D.; Wei, Y. Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.). Int. J. Mol. Sci. 2023, 24, 17221. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242417221
Liu C, Cheng H, Wang S, Yu D, Wei Y. Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.). International Journal of Molecular Sciences. 2023; 24(24):17221. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242417221
Chicago/Turabian StyleLiu, Congge, Haijing Cheng, Shuwei Wang, Dashi Yu, and Yunmin Wei. 2023. "Physiological and Transcriptomic Analysis Reveals That Melatonin Alleviates Aluminum Toxicity in Alfalfa (Medicago sativa L.)" International Journal of Molecular Sciences 24, no. 24: 17221. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms242417221