Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Media and Growth Conditions
2.2. Multiple Sequence Alignment of Fungal Arp1
2.3. Construction of Plasmids
2.4. Generation of the Smarp1 Deletion Strain ∆Smarp1
Name of Strain | Genotype | Reference |
---|---|---|
Sordaria macrospora | ||
DSM997 | S. macrospora wild-type (wt) | DSMZ |
S23442 | mutation in fus1-1 gene, brownish ascospores, fertile | [64] |
fus::RH2B | ectopic integration of pRH2B into S23442; hygR, pt, Pgpd::hh2b::tdTomato::TtrpC | [65] |
wt::TagRFP-T | ectopic integration of ptRFP_nat into DSM997; natR, ssi, Pccg1::TagRFP-T::TtrpC | [61] |
wt::GG-C-F-mNG | ectopic integration of pGG-C-F-mNG in DSM997; natR, ssi, Pgpd::mNG::3xFLAG::TtrpC | [59] |
∆Smku70 | ∆Smku70::natR, fertile | [66] |
Sm::Smarp1-TagRFP-T | ectopic integration of pSmarp1-TagRFP-T in DSM997; hygR, ssi, Smarp1P::Smarp1::TagRFP-T::TtrpC | this study |
Sm::Smarp1-mNG | ectopic integration of pSmarp1-mNG in DSM997; natR, ssi, Smarp1P::Smarp1::mNG::TtrpC | this study |
∆Smarp1 | ∆Smarp1::hygR, sterile | this study |
∆Smarp1::Smarp1 | ectopic integration of pSmarp1 in ∆Smarp1; hygR, natR, ssi, Smarp1P::Smarp1::Smarp1T | this study |
∆Smarp1::Smarp1-TagRFP-T | ectopic integration of pSmarp1-TagRFP-T in ∆Smarp1; hygR, natR, ssi, Smarp1P::Smarp1::TagRFP-T::TtrpC | this study |
Colletotrichum graminicola | ||
CgM2 | C. graminicola wild-type (wt); also referred to as M1.001 | [67] |
CgM2::Smarp1-mNG | ectopic integration of pSmarp1-mNG in CgM2; natR, ssi, Smarp1P::Smarp1::mNG::TtrpC | this study |
CgM2::Cgarp1-TagRFP-T | ectopic integration of pCgarp1-TagRFP-T in CgM2; genR, ssi, Cgarp1P::Cgarp1::TagRFP-T::TtrpC | this study |
2.5. Generation of S. macrospora Strains for Fluorescence Microscopy
2.6. Generation of C. graminicola Strains
2.7. Fluorescence Microscopy
2.8. Analysis of Chemotropic Growth Using the Arp1-TagRFP-T Marker
2.9. Protein Sample Preparation and Western Blot Hybridization
2.10. Phenotypic Analysis
3. Results
3.1. Arp1 Deletion in S. macrospora Results in a Severe Growth Defect That Can Only Partially Be Complemented
3.2. SmArp1 Localization Is a Dynamic Process at Growing Hyphal Tips and in Proximity to the Nucleus
3.3. Dynamic Localization of Arp1 in C. graminicola
3.4. Absence of CgArp1 localization to C. graminicola future fusion points
3.5. Arp1 Localizes Subapical to SPK
3.6. S. macrospora Arp1 Shows Dynamic Localization during Ascospore Germination
3.7. Analysis of Chemotropic Growth Is Improved by the Usage of the Arp1-TagRFP-T Marker
3.8. Tracking of Penetration Hyphae into Plant Tissue by Monitoring of Arp1 Localization in C. graminicola
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sm | Nc | Cg | Fo | Mo | An | Ro | Ml | Cc | Sco | Cn | Um | Pc | Sp | Ca | Sc | |
Sm | 100 | 100 | 93 | 92 | 88 | 78 | 74 | 74 | 70 | 73 | 71 | 74 | 74 | 51 | 56 | 50 |
Nc | 100 | 92 | 91 | 88 | 78 | 74 | 74 | 70 | 73 | 71 | 71 | 74 | 51 | 56 | 50 | |
Cg | 100 | 94 | 87 | 79 | 74 | 74 | 69 | 73 | 71 | 73 | 76 | 52 | 57 | 51 | ||
Fo | 100 | 87 | 78 | 74 | 74 | 70 | 74 | 72 | 74 | 75 | 51 | 57 | 50 | |||
Mo | 100 | 78 | 74 | 75 | 69 | 70 | 70 | 73 | 74 | 51 | 59 | 52 | ||||
An | 100 | 73 | 74 | 68 | 72 | 71 | 71 | 71 | 50 | 58 | 51 | |||||
Ro | 100 | 99 | 77 | 81 | 79 | 81 | 82 | 54 | 60 | 50 | ||||||
Ml | 100 | 77 | 82 | 78 | 82 | 81 | 54 | 60 | 50 | |||||||
Cc | 100 | 87 | 80 | 78 | 74 | 49 | 54 | 48 | ||||||||
Sco | 100 | 83 | 82 | 77 | 52 | 58 | 51 | |||||||||
Cn | 100 | 80 | 74 | 52 | 59 | 50 | ||||||||||
Um | 100 | 78 | 55 | 60 | 52 | |||||||||||
Pc | 100 | 56 | 58 | 51 | ||||||||||||
Sp | 100 | 47 | 44 | |||||||||||||
Ca | 100 | 53 | ||||||||||||||
Sc | 100 |
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Groth, A.; Schunke, C.; Reschka, E.J.; Pöggeler, S.; Nordzieke, D.E. Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes. J. Fungi 2021, 7, 580. https://0-doi-org.brum.beds.ac.uk/10.3390/jof7070580
Groth A, Schunke C, Reschka EJ, Pöggeler S, Nordzieke DE. Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes. Journal of Fungi. 2021; 7(7):580. https://0-doi-org.brum.beds.ac.uk/10.3390/jof7070580
Chicago/Turabian StyleGroth, Anika, Carolin Schunke, Eva Johanna Reschka, Stefanie Pöggeler, and Daniela Elisabeth Nordzieke. 2021. "Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes" Journal of Fungi 7, no. 7: 580. https://0-doi-org.brum.beds.ac.uk/10.3390/jof7070580