Physicochemical Characterization and Functional Analysis of the Polysaccharide from the Edible Microalga Nostoc sphaeroides
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physicochemical Characterization of Nostoglycan
2.2. Increase of Survival Rate and Reduction of ROS Levels by Nostoglycan in C. elegans under Oxidative Stress
2.3. Reduction of Protein Carbonyl and Malonaldehyde Contents and Upregulation of Antioxidant Enzyme Activities by Nostoglycan in C. elegans under Oxidative Stress
2.4. Inhibition of Proliferation of Human Tumor Cells by Nostoglycan
2.5. Induction of Apoptosis through Activation of Capspase-3 in Tumor Cells by Nostoglycan
3. Materials and Methods
3.1. Preparation of Polysaccharide
3.2. Determination of Carbohydrate Content and Analysis of Monosaccharide Composition
3.3. FTIR Spectroscopy Analysis
3.4. Congo Red Binding Assay
3.5. Surface Morphology Analysis
3.6. Assessment of Moisture Absorption and Retention
3.7. Determination of Viscosity
3.8. Nematode Maintenance
3.9. Paraquat Survival Assay in C. elegans
3.10. Determination of ROS Level in C. elegans
3.11. Determination of Protein Carbonyl and MDA Contents and Antioxidant Enzyme Activities in C. elegans
3.12. Tumor Cell Lines
3.13. Cell Viability Assay
3.14. Flow Cytometry Analysis of Cell Apoptosis
3.15. Measurement of Caspase-3 Activity
3.16. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Treatment | Protein Carbonyl Content a | MDA Content b | SOD Activity c | CAT Activity d | GPx Activity c |
---|---|---|---|---|---|
Control | 0.88 ± 0.15 | 7.60 ± 0.54 | 49.02 ± 4.66 | 1.20 ± 0.02 | 15.59 ± 1.63 |
Nostoglycan | 0.82 ± 0.12 | 4.94 ± 0.31 e | 46.81 ± 6.84 | 1.32 ± 0.02 e | 16.87 ± 1.50 |
Paraquat | 1.71 ± 0.14 e | 10.39 ± 0.87 e | 69.97 ± 3.51 e | 1.08 ± 0.02 e | 15.09 ± 0.76 |
Nostoglycan + Paraquat | 1.02 ± 0.18 f | 7.28 ± 0.46 f | 97.88 ± 6.72 f | 1.21 ± 0.02 f | 14.19 ± 1.48 |
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Li, H.; Su, L.; Chen, S.; Zhao, L.; Wang, H.; Ding, F.; Chen, H.; Shi, R.; Wang, Y.; Huang, Z. Physicochemical Characterization and Functional Analysis of the Polysaccharide from the Edible Microalga Nostoc sphaeroides. Molecules 2018, 23, 508. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23020508
Li H, Su L, Chen S, Zhao L, Wang H, Ding F, Chen H, Shi R, Wang Y, Huang Z. Physicochemical Characterization and Functional Analysis of the Polysaccharide from the Edible Microalga Nostoc sphaeroides. Molecules. 2018; 23(2):508. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23020508
Chicago/Turabian StyleLi, Haifeng, Linnan Su, Sheng Chen, Libin Zhao, Hongyu Wang, Fei Ding, Hong Chen, Ruona Shi, Yulan Wang, and Zebo Huang. 2018. "Physicochemical Characterization and Functional Analysis of the Polysaccharide from the Edible Microalga Nostoc sphaeroides" Molecules 23, no. 2: 508. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23020508