Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li.
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of Single-Factor by Ultrasound-assisted Extraction on Extraction Yield and Extraction Rate of Polysaccharides from Auricularia cornea var. Li.
2.2. Effects of Single-Factor by Hot Water Extraction on Extraction Yield and Extraction Rate of Polysaccharides from Auricularia cornea var. Li.
2.3. Extraction Yield, Extraction Rate, Purity of Polysaccharides by Ultrasound-assisted Extraction and Hot Water extraction
2.4. Microstructure
2.5. Fourier Transform Infrared Spectra
2.6. Rheological Properties
2.6.1. Steady-Shear Flow Property
2.6.2. Dynamic Viscoelastic Property
3. Materials and Methods
3.1. Materials
3.2. Extraction of Polysaccharides from Auricularia cornea var. Li.
3.2.1. Ultrasound-assisted Extraction of Polysaccharides from Auricularia cornea var. Li.
3.2.2. Hot Water Extraction of Polysaccharides from Auricularia cornea var. Li.
3.2.3. Calculation of Extraction Yield, Extraction Rate, Purity
3.3. Scanning Electron Microscopy Analysis
3.4. Fourier Transform Infrared Spectroscopy Analysis
3.5. Rheological Property Measurements
3.5.1. Sample Preparation
3.5.2. Steady-Shear Flow Property
3.5.3. Dynamic Viscoelastic Property
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
- Xue, Z.; Hao, J.; Yu, W.; Kou, X. Effects of Processing and Storage Preservation Technologies on Nutritional Quality and Biological Activities of Edible Fungi: A Review. J. Food Process. Eng. 2017, 40, e12437. [Google Scholar] [CrossRef]
- Zeng, W.C.; Zhang, Z.; Gao, H.; Jia, L.R.; Chen, W.Y. Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction. Carbohydr. Polym. 2012, 89, 694–700. [Google Scholar] [CrossRef] [PubMed]
- Jeff, I.B.; Li, S.; Peng, X.; Kassim, R.M.; Liu, B.; Zhou, Y. Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes. Fitoterapia 2013, 84, 338–346. [Google Scholar] [CrossRef] [PubMed]
- Ma, G.; Yang, W.; Mariga, A.M.; Fang, Y.; Ma, N.; Pei, F.; Hu, Q. Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue. Carbohydr. Polym. 2014, 114, 297–305. [Google Scholar] [CrossRef] [PubMed]
- Yang, W.; Pei, F.; Shi, Y.; Zhao, L.; Fang, Y.; Hu, Q. Purification, characterization and anti-proliferation activity of polysaccharides from Flammulina velutipes. Carbohydr. Polym. 2012, 88, 474–480. [Google Scholar] [CrossRef]
- Ye, M.; Qiu, T.; Peng, W.; Chen, W.-X.; Ye, Y.-W.; Lin, Y.-R. Purification, characterization and hypoglycemic activity of extracellular polysaccharides from Lachnum calyculiforme. Carbohydr. Polym. 2011, 86, 285–290. [Google Scholar] [CrossRef]
- Zhu, Y.; Li, Q.; Mao, G.; Zou, Y.; Feng, W.; Zheng, D.; Wang, W.; Zhou, L.; Zhang, T.; Yang, J.; et al. Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus. Carbohydr. Polym. 2014, 101, 606–613. [Google Scholar] [CrossRef] [PubMed]
- Wang, J.; Zhang, J.; Zhao, B.; Wang, X.; Wu, Y.; Yao, J. A comparison study on microwave-assisted extraction of Potentilla anserina L. polysaccharides with conventional method: Molecule weight and antioxidant activities evaluation. Carbohydr. Polym. 2010, 80, 84–93. [Google Scholar] [CrossRef]
- Cheong, K.L.; Wang, L.Y.; Wu, D.T.; Hu, D.J.; Zhao, J.; Li, S.P. Microwave-Assisted Extraction, Chemical Structures, and Chain Conformation of Polysaccharides from a Novel Cordyceps Sinensis Fungus UM01. J. Food Sci. 2016, 81, C2167–C2174. [Google Scholar] [CrossRef] [PubMed]
- Tian, Y.; Zeng, H.; Xu, Z.; Zheng, B.; Lin, Y.; Gan, C.; Lo, Y.M. Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus). Carbohydr. Polym. 2012, 88, 522–529. [Google Scholar] [CrossRef]
- Chiang, C.F.; Lai, L.S. Effect of enzyme-assisted extraction on the physicochemical properties of mucilage from the fronds of Asplenium australasicum (J. Sm.) Hook. Int. J. Biol. Macromol. 2019, 124, 346–353. [Google Scholar] [CrossRef] [PubMed]
- Chen, Y.; Yao, F.; Ming, K.; Wang, D.; Hu, Y.; Liu, J. Polysaccharides from Traditional Chinese Medicines: Extraction, Purification, Modification, and Biological Activity. Molecules 2016, 21, 1705. [Google Scholar] [CrossRef] [PubMed]
- You, Q.; Yin, X.; Zhang, S.; Jiang, Z. Extraction, purification, and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai. Carbohydr. Polym. 2014, 99, 1–10. [Google Scholar] [CrossRef] [PubMed]
- Youssouf, L.; Lallemand, L.; Giraud, P.; Soule, F.; Bhaw-Luximon, A.; Meilhac, O.; D’Hellencourt, C.L.; Jhurry, D.; Couprie, J. Ultrasound-assisted extraction and structural characterization by NMR of alginates and carrageenans from seaweeds. Carbohydr. Polym. 2017, 166, 55–63. [Google Scholar] [CrossRef] [PubMed]
- Poommarinvarakul, S.; Tattiyakul, J.; Muangnapoh, C. Isolation and rheological properties of tamarind seed polysaccharide from tamarind kernel powder using protease enzyme and high-intensity ultrasound. J. Food Sci. 2010, 75, E253–E260. [Google Scholar] [CrossRef] [PubMed]
- Ying, Z.; Han, X.; Li, J. Ultrasound-assisted extraction of polysaccharides from mulberry leaves. Food Chem. 2011, 127, 1273–1279. [Google Scholar] [CrossRef] [PubMed]
- Ma, F.; Zhang, Y.; Liu, N.; Zhang, J.; Tan, G.; Kannan, B.; Liu, X.; Bell, A.E. Rheological properties of polysaccharides from Dioscorea opposita Thunb. Food Chem. 2017, 227, 64–72. [Google Scholar] [CrossRef] [PubMed]
- Wang, W.; Li, C.; Du, G.; Zhang, X.; Zhang, H. Characteristics and Rheological Properties of Polysaccharide Nanoparticles from Edible Mushrooms (Flammulina velutipes). J. Food Sci. 2017, 82, 687–693. [Google Scholar] [CrossRef] [PubMed]
- Bao, H.; You, S.; Cao, L.; Zhou, R.; Wang, Q.; Cui, S.W. Chemical and rheological properties of polysaccharides from fruit body of Auricularia auricular-judae. Food Hydrocoll. 2016, 57, 30–37. [Google Scholar] [CrossRef]
- Zhong, K.; Zhang, Q.; Tong, L.; Liu, L.; Zhou, X.; Zhou, S. Molecular weight degradation and rheological properties of schizophyllan under ultrasonic treatment. Ultrason. Sonochem. 2015, 23, 75–80. [Google Scholar] [CrossRef] [PubMed]
- Li, J.; Li, B.; Geng, P.; Song, A.-X.; Wu, J.-Y. Ultrasonic degradation kinetics and rheological profiles of a food polysaccharide (konjac glucomannan) in water. Food Hydrocoll. 2017, 70, 14–19. [Google Scholar] [CrossRef]
- Seshadri, R.; Weiss, J.; Hulbert, G.J.; Mount, J.J.F.H. Ultrasonic processing influences rheological and optical properties of high-methoxyl pectin dispersions. Food Hydrocoll. 2003, 17, 191–197. [Google Scholar] [CrossRef]
- Zhao, C.; Liao, Z.; Wu, X.; Liu, Y.; Liu, X.; Lin, Z.; Huang, Y.; Liu, B. Isolation, purification, and structural features of a polysaccharide from Phellinus linteus and its hypoglycemic effect in alloxan-induced diabetic mice. J. Food Sci. 2014, 79, H1002–H1010. [Google Scholar] [CrossRef] [PubMed]
- Xiao, L.; Kuotan, Z. A review of the advances of the research on a white variant strain in Genus Auricularia. Edible Med. Mushrooms 2016, 24, 230–233. (In Chinese) [Google Scholar]
- Yuchun, C.; Haiying, B.; Xiao, L.; Tolgor, B.; Yu, L. Anti-tumor activities of Auricularia cornea fruiting body extract in H22 bearing mice. Mycosystema 2017, 26, 1289–1298. (In Chinese) [Google Scholar] [CrossRef]
- Miao, Y.-Z.; Lin, Q.; Cao, Y.; He, G.-H.; Qiao, D.-R.; Cao, Y. Extraction of water-soluble polysaccharides (WSPS) from Chinese truffle and its application in frozen yogurt. Carbohydr. Polym. 2011, 86, 566–573. [Google Scholar] [CrossRef]
- XuJie, H.; Wei, C. Optimization of extraction process of crude polysaccharides from wild edible BaChu mushroom by response surface methodology. Carbohydr. Polym. 2008, 72, 67–74. [Google Scholar] [CrossRef]
- Ponmurugan, K.; Al-Dhabi, N.A.; Maran, J.P.; Karthikeyan, K.; Moothy, I.G.; Sivarajasekar, N.; Manoj, J.J.B. Ultrasound assisted pectic polysaccharide extraction and its characterization from waste heads of Helianthus annus. Carbohydr. Polym. 2017, 173, 707–713. [Google Scholar] [CrossRef] [PubMed]
- Hu, H.; Zhao, Q.; Pang, Z.; Xie, J.; Lin, L.; Yao, Q. Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace. Int. J. Biol. Macromol. 2018, 117, 1314–1325. [Google Scholar] [CrossRef] [PubMed]
- Zhao, Z.; Xu, X.; Ye, Q.; Dong, L. Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity. Int. J. Biol. Macromol. 2013, 59, 290–294. [Google Scholar] [CrossRef] [PubMed]
- Chen, C.; You, L.J.; Abbasi, A.M.; Fu, X.; Liu, R.H. Optimization for ultrasound extraction of polysaccharides from mulberry fruits with antioxidant and hyperglycemic activity in vitro. Carbohydr. Polym. 2015, 130, 122–132. [Google Scholar] [CrossRef] [PubMed]
- Fu, L.; Chen, H.; Dong, P.; Zhang, X.; Zhang, M. Effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from mushroom Inonotus obliquus. J. Food Sci. 2010, 75, C322–C327. [Google Scholar] [CrossRef] [PubMed]
- Sahin, S.; Samli, R. Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology. Ultrason. Sonochem. 2013, 20, 595–602. [Google Scholar] [CrossRef] [PubMed]
- Hou, F.; Wu, Y.; Kan, L.; Li, Q.; Xie, S.; Ouyang, J. Effects of Ultrasound on the Physicochemical Properties and Antioxidant Activities of Chestnut Polysaccharide. Int. J. Food Eng. 2016, 12, 439–449. [Google Scholar] [CrossRef]
- Prakash Maran, J.; Manikandan, S.; Thirugnanasambandham, K.; Vigna Nivetha, C.; Dinesh, R. Box-Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide. Carbohydr. Polym. 2013, 92, 604–611. [Google Scholar] [CrossRef] [PubMed]
- Shen, X.; Fang, T.; Gao, F.; Guo, M. Effects of ultrasound treatment on physicochemical and emulsifying properties of whey proteins pre- and post-thermal aggregation. Food Hydrocoll. 2017, 63, 668–676. [Google Scholar] [CrossRef]
- Qiao, D.; Hu, B.; Gan, D.; Sun, Y.; Ye, H.; Zeng, X. Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii. Carbohydr. Polym. 2009, 76, 422–429. [Google Scholar] [CrossRef]
- Zhao, C.; Li, X.; Miao, J.; Jing, S.; Li, X.; Huang, L.; Gao, W. The effect of different extraction techniques on property and bioactivity of polysaccharides from Dioscorea hemsleyi. Int. J. Biol. Macromol. 2017, 102, 847–856. [Google Scholar] [CrossRef] [PubMed]
- Boulet, J.C.; Williams, P.; Doco, T. A Fourier transform infrared spectroscopy study of wine polysaccharides. Carbohydr. Polym. 2007, 69, 79–85. [Google Scholar] [CrossRef]
- Jahanbin, K.; Abbasian, A.; Ahang, M. Isolation, purification and structural characterization of a new water-soluble polysaccharide from Eremurus stenophyllus (boiss. & buhse) baker roots. Carbohydr. Polym. 2017, 178, 386–393. [Google Scholar] [CrossRef] [PubMed]
- Maran, J.P.; Priya, B. Ultrasound-assisted extraction of polysaccharide from Nephelium lappaceum L. fruit peel. Int. J. Biol. Macromol. 2014, 70, 530–536. [Google Scholar] [CrossRef] [PubMed]
- Wang, Q.H.; Shu, Z.P.; Xu, B.Q.; Xing, N.; Jiao, W.J.; Yang, B.Y.; Kuang, H.X. Structural characterization and antioxidant activities of polysaccharides from Citrus aurantium L. Int. J. Biol. Macromol. 2014, 67, 112–123. [Google Scholar] [CrossRef] [PubMed]
- Wu, W.; Zhu, Y.; Zhang, L.; Yang, R.; Zhou, Y. Extraction, preliminary structural characterization, and antioxidant activities of polysaccharides from Salvia miltiorrhiza Bunge. Carbohydr. Polym. 2012, 87, 1348–1353. [Google Scholar] [CrossRef] [Green Version]
- Guo, X.; Shang, X.; Zhou, X.; Zhao, B.; Zhang, J. Ultrasound-assisted extraction of polysaccharides from Rhododendron aganniphum: Antioxidant activity and rheological properties. Ultrason. Sonochem. 2017, 38, 246–255. [Google Scholar] [CrossRef] [PubMed]
- Tabarsa, M.; Anvari, M.; Joyner, H.S.; Behnam, S.; Tabarsa, A. Rheological behavior and antioxidant activity of a highly acidic gum from Althaea officinalis flower. Food Hydrocoll. 2017, 69, 432–439. [Google Scholar] [CrossRef]
- Guo, X.; Ye, X.; Sun, Y.; Wu, D.; Wu, N.; Hu, Y.; Chen, S. Ultrasound effects on the degradation kinetics, structure, and antioxidant activity of sea cucumber fucoidan. J. Agric. Food. Chem. 2014, 62, 1088–1095. [Google Scholar] [CrossRef] [PubMed]
- Zhang, L.; Ye, X.; Ding, T.; Sun, X.; Xu, Y.; Liu, D. Ultrasound effects on the degradation kinetics, structure and rheological properties of apple pectin. Ultrason. Sonochem. 2013, 20, 222–231. [Google Scholar] [CrossRef] [PubMed]
- Zeng, H.; Zhang, Y.; Jian, Y.; Tian, Y.; Miao, S.; Zheng, B. Rheological properties, molecular distribution, and microstructure of Fortunella margarita (Lour.) swingle polysaccharides. J. Food Sci. 2015, 80, E742–E749. [Google Scholar] [CrossRef] [PubMed]
- Shobha, M.S.; Tharanathan, R.N. Rheological behaviour of pullulanase-treated guar galactomannan on co-gelation with xanthan. Food Hydrocoll. 2009, 23, 749–754. [Google Scholar] [CrossRef]
- Shen, X.; Zhao, C.; Guo, M. Effects of high intensity ultrasound on acid-induced gelation properties of whey protein gel. Ultrason. Sonochem. 2017, 39, 810–815. [Google Scholar] [CrossRef] [PubMed]
- Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; Smith, F.J.A.C. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28, 350–356. [Google Scholar] [CrossRef]
- Sun, X.; Wang, C.; Wang, H.; Guo, M. Effects of Processing on Structure and Thermal Properties of Powdered Preterm Infant Formula. J. Food Sci. 2018, 83, 1685–1694. [Google Scholar] [CrossRef] [PubMed]
- Wei, Y.; Lin, Y.; Xie, R.; Xu, Y.; Yao, J.; Zhang, J. The flow behavior, thixotropy and dynamical viscoelasticity of fenugreek gum. J. Food Eng. 2015, 166, 21–28. [Google Scholar] [CrossRef]
- Zheng, J.; Zeng, R.; Kan, J.; Zhang, F. Effects of ultrasonic treatment on gel rheological properties and gel formation of high-methoxyl pectin. J. Food Eng. 2018, 231, 83–90. [Google Scholar] [CrossRef]
Methods | HWE | UAE |
---|---|---|
Particle size (mesh) | 150–200 | 150–200 |
Water to raw material ratio (mL/g) | 60:1 | 70:1 |
Extraction time | 3.0 h | 40 min |
Extraction temperature (°C) | 90 | 70 |
Ultrasonic amplitude (%) | / | 40 |
Extraction yield of polysaccharides (%) | 30.35 ± 1.67 a | 30.99 ± 1.93 a |
Extraction rate of polysaccharides (%) | 24.95 ± 2.78 a | 29.29 ± 1.41 b |
Purity of polysaccharides (%) | 75.33 ± 6.15 a | 88.62 ± 2.80 b |
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Wang, Y.; Wang, C.; Guo, M. Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li. Molecules 2019, 24, 939. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050939
Wang Y, Wang C, Guo M. Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li. Molecules. 2019; 24(5):939. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050939
Chicago/Turabian StyleWang, Yinping, Cuina Wang, and Mingruo Guo. 2019. "Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li." Molecules 24, no. 5: 939. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050939