Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of Bacteria
2.2. Petroleum-Hydrocarbon Degradation Potential
2.3. Plant Growth-Promoting Traits
3. Discussion
4. Materials and Methods
4.1. Site Description, Experimental Design and Sample Collection
4.2. Bacterial Isolation
4.3. DNA Extraction, PCR Amplification, and Sequencing of Bacterial Isolates
4.4. Nucleotide Sequence Analyses and Accession Numbers
4.5. Assessment of Hydrocarbon Degradation Potential of the Bacterial Isolates
4.6. Assessment of Plant Growth-Promoting (PGP) Traits of the Bacterial Isolates
4.6.1. Phosphate Solubilization
4.6.2. Screening for Nitrogen Fixation
4.6.3. ACC Deaminase Activity
4.6.4. Indole-3- Acetic Acid (IAA) Production
4.6.5. Siderophore Production
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Serial # | Genus1 | Eleocharis Rhizosphere 2 | Bulk Soil 2 | Salix Rhizosphere 2 |
---|---|---|---|---|
1 | Acidovorax | 1 | 0 | 0 |
2 | Acinetobacter | 3 | 15 | 0 |
3 | Aeromonas | 4 | 0 | 0 |
4 | Agrococcus | 0 | 1 | 0 |
5 | Agromyces | 2 | 3 | 0 |
6 | Amycolatopsis | 0 | 0 | 2 |
7 | Ancylobacter | 1 | 0 | 0 |
8 | Arthrobacter | 0 | 0 | 20 |
9 | Azorhizobium | 2 | 0 | 0 |
10 | Bacillus | 8 | 16 | 17 |
11 | Bosea | 2 | 0 | 0 |
12 | Brevibacillus | 0 | 1 | 0 |
13 | Brevundimonas | 0 | 2 | 0 |
14 | Caulobacter | 0 | 0 | 1 |
15 | Chitinimonas | 2 | 0 | 9 |
16 | Chryseobacterium | 2 | 2 | 0 |
17 | Citrobacter | 0 | 2 | 1 |
18 | Comamonas | 1 | 0 | 0 |
19 | Delftia | 3 | 0 | 0 |
20 | Dyella | 1 | 3 | 0 |
21 | Empedobacter | 1 | 2 | 0 |
22 | Enterobacter | 1 | 3 | 3 |
23 | Exiguobacterium | 2 | 2 | 0 |
24 | Flavihumibacter | 0 | 1 | 0 |
25 | Flavobacteriaceae | 1 | 0 | 0 |
26 | Georgenia | 0 | 1 | 0 |
27 | Gordonia | 1 | 1 | 3 |
28 | Hydrogenophaga | 0 | 1 | 0 |
29 | Klebsiella | 11 | 7 | 3 |
30 | Luteibacter | 0 | 1 | 0 |
31 | Lysinibacillus | 0 | 0 | 1 |
32 | Lysinimonas | 2 | 0 | 0 |
33 | Massilia | 0 | 4 | 1 |
34 | Mesorhizobium | 0 | 0 | 1 |
35 | Microbacterium | 13 | 9 | 1 |
36 | Micromonospora | 0 | 0 | 2 |
37 | Mycobacterium | 1 | 0 | 0 |
38 | Mycolicibacterium | 2 | 3 | 2 |
39 | Myroides | 3 | 2 | 0 |
40 | Nocardia | 0 | 0 | 2 |
41 | Nocardioides | 0 | 0 | 9 |
42 | Paenarthrobacter | 0 | 0 | 2 |
43 | Paenibacillus | 0 | 0 | 1 |
44 | Pantoea | 1 | 1 | 1 |
45 | Phycicoccus | 0 | 0 | 2 |
46 | Pseudarthrobacter | 0 | 0 | 2 |
47 | Pseudomonas | 36 | 11 | 15 |
48 | Pseudoxanthomonas | 5 | 12 | 0 |
49 | Raoultella | 0 | 0 | 6 |
50 | Rheinheimera | 1 | 2 | 0 |
51 | Rhizobium | 4 | 1 | 0 |
52 | Rhodococcus | 2 | 6 | 2 |
53 | Rhodospirillum | 1 | 0 | 0 |
54 | Serratia | 5 | 0 | 0 |
55 | Sphingobacterium | 0 | 2 | 0 |
56 | Sphingobium | 0 | 1 | 0 |
57 | Sphingomonas | 1 | 1 | 1 |
58 | Sphingopyxis | 2 | 1 | 0 |
59 | Staphylococcus | 0 | 1 | 1 |
60 | Stenotrophomonas | 3 | 8 | 1 |
61 | Streptomyces | 1 | 0 | 32 |
62 | Unidentified bacteria | 7 | 14 | 0 |
63 | Variovorax | 1 | 0 | 12 |
Serial # | Isolate | Closest NCBI Relative | Environmental Niche | Isolation Medium |
---|---|---|---|---|
1 | SB41 | Acinetobacter calcoaceticus | Bulk soil | B-H_amended diesel |
2 | SB55 | Acinetobacter calcoaceticus | Bulk soil | B-H_amended diesel |
3 | SB60 | Acinetobacter sp. | Bulk soil | B-H_amended diesel |
4 | ET27 | Pseudomonas plecoglossicida | Eleocharis rhizosphere | TSA |
5 | ET43 | Serratia sp. | Eleocharis rhizosphere | TSA |
6 | ET45 | Pseudomonas fluorescens | Eleocharis rhizosphere | TSA |
7 | ET46 | Serratia sp. | Eleocharis rhizosphere | TSA |
8 | ET50 | Pseudomonas putida | Eleocharis rhizosphere | TSA |
9 | ET52 | Serratia sp. | Eleocharis rhizosphere | TSA |
10 | ET57 | Pseudomonas monteilii | Eleocharis rhizosphere | TSA |
11 | ET60 | Azomonas macrocytogenes | Eleocharis rhizosphere | TSA |
12 | EB3 | Pseudomonas songnenensis | Eleocharis rhizosphere | B-H_amended diesel |
13 | EB31 | Stenotrophomonas pavanii | Eleocharis rhizosphere | B-H_amended diesel |
14 | WT4 | Pseudomonas mandelii | Salix rhizosphere | 1/10TSA |
15 | WT8 | Streptomyces atriruber | Salix rhizosphere | 1/10TSA |
16 | WT17 | Pseudomonas kilonensis | Salix rhizosphere | 1/10TSA |
17 | WT22 | Pseudomonas frederiksbergensis | Salix rhizosphere | 1/10TSA |
18 | WT32 | Bacillus megaterium | Salix rhizosphere | 1/10TSA |
19 | WT50 | Pseudomonas frederiksbergensis | Salix rhizosphere | 1/10TSA |
20 | WT56 | Pseudomonas frederiksbergensis | Salix rhizosphere | 1/10TSA |
21 | WB31 | Pseudomonas putida | Salix rhizosphere | B-H_amended diesel |
22 | EA21 | Pantoea agglomerans | Eleocharis rhizosphere | ACCD |
pH (1:1) | CEC (meq/100 g) | N (g/Kg) | P (Kg/ha) | K (Kg/ha) | Ca (Kg/ha) | Mg (kg/ha) | Mn (PPM) | O.M (%) | Fe (PPM) | |
---|---|---|---|---|---|---|---|---|---|---|
Salix rhizosphere | 7.4 | 24.8 | 0.9 | <10 | 453 | 7323 | 2127 | 34.5 | 3.5 | 291.79 |
Eleocharis Rhizosphere | 7.3 | 38.7 | 2.2 | <10 | 566 | 13961 | 1851 | 61.5 | 6.2 | 582.56 |
Bulk soil | 7.4 | 37.2 | 2.1 | 13 | 565 | 13121 | 1952 | 99.7 | 7.5 | 580.77 |
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Alotaibi, F.; Lee, S.-J.; St-Arnaud, M.; Hijri, M. Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties. Plants 2021, 10, 1987. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101987
Alotaibi F, Lee S-J, St-Arnaud M, Hijri M. Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties. Plants. 2021; 10(10):1987. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101987
Chicago/Turabian StyleAlotaibi, Fahad, Soon-Jae Lee, Marc St-Arnaud, and Mohamed Hijri. 2021. "Salix purpurea and Eleocharis obtusa Rhizospheres Harbor a Diverse Rhizospheric Bacterial Community Characterized by Hydrocarbons Degradation Potentials and Plant Growth-Promoting Properties" Plants 10, no. 10: 1987. https://0-doi-org.brum.beds.ac.uk/10.3390/plants10101987