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Review

What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?

by
Paulina Marciniak
and
Justyna Możejko-Ciesielska
*
Department of Microbiology and Mycology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10719 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Polymers 2021, 13(11), 1731; https://0-doi-org.brum.beds.ac.uk/10.3390/polym13111731
Submission received: 29 April 2021 / Revised: 21 May 2021 / Accepted: 22 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Production of Polyhydroxyalkanoate (PHA) Biopolymers from Waste Materials)
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Abstract

:
The rising global consumption and industrialization has resulted in increased food processing demand. Food industry generates a tremendous amount of waste which causes serious environmental issues. These problems have forced us to create strategies that will help to reduce the volume of waste and the contamination to the environment. Waste from food industries has great potential as substrates for value-added bioproducts. Among them, polyhydroxyalkanaotes (PHAs) have received considerable attention in recent years due to their comparable characteristics to common plastics. These biodegradable polyesters are produced by microorganisms during fermentation processes utilizing various carbon sources. Scale-up of PHA production is limited due to the cost of the carbon source metabolized by the microorganisms. Therefore, there is a growing need for the development of novel microbial processes using inexpensive carbon sources. Such substrates could be waste generated by the food industry and food service. The use of industrial waste streams for PHAs biosynthesis could transform PHA production into cheaper and more environmentally friendly bioprocess. This review collates in detail recent developments in the biosynthesis of various types of PHAs produced using waste derived from agrofood industries. Challenges associated with this production bioprocess were described, and new ways to overcome them were proposed.

1. Introduction

Synthetic polymers are distinguished by such features as a high level of strength, durability and lightness. Moreover, they could be easily chemically modified which makes them versatile and innovative materials used in countless products from nearly all industrial sectors. However, production of petrochemical plastics has led to environmental problems because they are accumulated in the most varied environments and are not easily degradable. Worldwide production of plastics is still increasing reaching 359 million tons in 2018, so 155 million tons more compared to 2002 [1]. In addition, in spite of many efforts to increase the amount of plastic waste sent to recycling, only 32.5% were recycled in Europe in 2018. The remaining plastics are discarded in the landfills, left in the open dumps or in the natural environment. During their decomposition process, microplastics (MPs) are formed which have negative impacts on the environment that have become a global concern. Particles of microplastics can easily enter into the interior of the body by water or food and be transferred through the food chain to other organisms [2]. Therefore, the synthetic polymers need to be substituted by their ecofriendly alternatives. Furthermore, biobased substitutes for petrochemical polymers that are generated in microbial processes using renewable resources can reduce emissions of greenhouse gases, and the materials produced based on the biopolymers are easier to recycle to the virgin polymer and, at the end of their useful life, biodegrade in the environment [3].
Among the various groups of biopolymers, polyhydroxyalkanoates (PHAs) can be used to replace a wide variety of traditional plastics. They are synthesized and accumulated by microorganisms in their cytoplasm in the form of granules [4]. The synthesis of PHAs in bacterial cells takes place under unbalanced growth conditions when carbon source is present in excess and nutrients such as nitrogen or phosphorus are in deficiency [5]. Due to biodegradability and biocompatibility, PHAs have gained much attention of industry in recent years. It is predicted that the bioplastic industry will be a key player on the materials market in the future. The production of PHAs from commercial manufacturers reached 2.05 million tons in 2017. It is believed that the global market of PHAs will grow up to 98 million tons by 2024 [6]. Many companies have been set up to commercialize PHAs as environmentally friendly bioplastics using renewable resources as primary or secondary feedstocks (Table 1). However, the deployment of PHAs into the global market is still in its early stages. Because of their high production costs, PHAs wide commercialization and industrialization is limited. Therefore, there is a need to employ cheaper substrates that will be used for bacterial growth and will support PHAs synthesis and accumulation. In order to decrease costs and make PHAs production bioprocess more economically feasible, much research has been conducted using industrial waste streams for microbial fermentation processes. On the other hand, the use of waste carbon sources for microbial-derived PHAs production could reduce waste disposable costs [7].
Due to the rising global population and, as a consequence, increased food processing demand, a tremendous amount of food waste is generated, causing serious environmental problems. Recently, great efforts are made to convert food waste into value-added products such as biohydrogen, volatile fatty acids [15], bioactive compounds [16] or biopolymers [17]. Despite various types of waste that can be used as substrates for PHAs production, recently more attention has been focused on the agri-food wastes which have a high rate of production worldwide. These wastes seem to be potential raw materials for bacterial fermentation to produce value-added PHAs being a good strategy not only for the promotion of the waste’s recovery but also the development of desirable bioproducts.
The aim of this review is to discuss current knowledge on the use of agro-industrial wastes as carbon sources for PHAs production. Moreover, the challenges and prospects of using them in the fermentation processes for PHAs industrial scale production will be presented. The recent review published by Kumar et al. [18] on the similar topic was focused on the economy of PHAs production by bacteria, their extraction and applications, whereas the presented article described comprehensively the latest progress in PHAs production using wild-type and recombinant bacteria.

2. PHAs Characterization and Biosynthesis

The chain of PHAs consists of from 600 to 35,000 hydroxy fatty acid monomer units. It is polyester in which the side chain R group is usually a saturated alkyl group. Depending on the total number of carbon atoms in a monomer, PHAs are divided into three groups: short-chain-length PHAs (PHASCL) which contain from 3 to 5 carbon atoms, medium-chain-length PHAs (PHAMCL) with 6–14 carbon atoms in each unit and long-chain-length PHAs (PHALCL) which contain more than 15 carbon atoms [5]. PHAs are characterized by several useful traits. PHASCL like poly(3-hydroxybutyrate) [(P(3HB)] is a highly crystalline polymer with high brittleness, high melting temperature and glass transition temperature, and with poor elastic properties. They are produced by many bacterial species such as Bacillus megaterium, Cupriavidus necator, Aeromonas hydrophila. PHAMCL like poly(3-hydroxyhexanoate) [P(3HHx)] possess more desirable properties. They have low degree of crystallinity, low tensile strength, high elongation to break, low melting temperature and high elasticity. PHAMCL are synthesized mainly by Pseudomonas species. PHALCL like poly(3-hydroxyhexadecanoate) [(P(3HHxD)] is uncommon and knowledge about its properties and potential applications is still limited. The most important feature of all types of PHAs is their ability to be degraded after being discarded. The degradation of these biopolymers lasts up to one year and is a result of the action of extracellular enzymes called PHA depolymerases synthesized by some bacteria and fungi [19]. It is well known that the efficiency of their decomposition is dependent on several factors, e.g., activity of microorganisms, environmental conditions, temperature and PHAs properties like molecular weight [20]. Moreover, unlike synthetic polymers like polypropylene, PHAs are hydrophobic, nontoxic, insoluble in water and more resistant to UV radiation [21]. These properties enable their wide range of application (Table 2).
Recent advances in the biodegradability of PHAs have been comprehensively described by Meereboer et al. [22]. It is known that PHA-degrading bacteria could be divided into two groups: microorganisms that physically degrade PHAs and those which metabolize the by-products of PHAs degradation. PHAs are considered the most readily degradable biopolymer both in aerobic and anaerobic conditions compared to other biopolymers such as polylactide, polybutylene succinate or polybutyrate adipate terephthalate. However, taking into account the industrial applications based on PHA monomers the addition of additives or other biodegradable/nonbiodegradable polymers is needed to improve their properties. It was reported that antifouling agents or plasticizers influence negatively on the PHA biodegradation process [23]. On the other hand, incorporation of natural fibers could improve the rate of PHA biodegradation [24]. Therefore, the research in the area of biodegradation processes of PHA biocomposites should be conducted to understand deeper a biodegradable nature of PHAs.
It is estimated that up to 150 bacterial genera are capable of producing PHAs [25]. PHAs-producing bacteria that are able to grow on waste feedstocks include those commonly found in the environment, e.g., in soil [26,27]. Recent studies indicated the potential of marine bacteria to PHAs synthesis, among them the special attention has gained Halomonas sp., Salinivibrio sp. [28,29,30,31].
PHA synthases are the key enzymes involved in the polymerization of PHAs using the (R)-3-hydroxyacyl-CoA as a substrate. They are divided into four class depending on their subunit composition, amino acid sequence, and substrate specificity. PHA synthases belong to group I, III, and IV are found to polymerize short-chain-length monomers, and group II medium-chain-length monomers. Group I and group II PHA synthases contain a single PhaC subunit with molecular mass between 61 and 73 kDa; they were found in Cupriavidus necator and Pseudomonas species, respectively. Group III and IV synthases consist of two subunits. Group III synthase has PhaC and PhaE subunits, whereas group IV PhaC and PhaR. The class III and class IV of PHA synthases were observed in Allochromatium vinosum and Bacillus megaterium, respectively [41]. The synthesis of polyhydroxyalkanoates by bacteria under laboratory conditions enforces maintaining appropriate conditions such as the proper concentration of carbon to nitrogen, the presence of macro and microelements, the optimum temperature and pH, the proper concentration of inoculum [42]. Moreover, researchers reported that unbalanced growth conditions are more favorable to efficient PHAs synthesis and accumulation [43,44]. However, a bacterial species produced the PHAs in the biotechnological processes is the most important factor. Many microorganisms have been studied for PHA synthesis, but only a few of them have a promising ability to produce these biopolyesters. Many bacteria did not meet the crucial criteria, such as the capability to metabolize raw carbon sources to achieve a satisfied growth rate and a high PHAs productivity (Figure 1).
Furthermore, to compete with synthetic plastics the overall PHAs production costs should be decreased by using inexpensive feedstocks. Therefore, many scientific groups have focused their attention on the possibility of using agro-industrial waste streams for fermentation processes to make PHAs economically feasible [45]. A number of bacterial species have been reported to synthesize PHAs in satisfactory amounts utilizing waste from the various agrofood industry (Table 3).

3. PHAs Biosynthesis Using By-Products of the Dairy Industry

The global production of by-products from the dairy industry reached 180–190 million tons per year [83]. During cheese production, whey as a byproduct is generated in high volumes. It is a waste liquid derived from cheese manufacturing that seems to be an appropriate substrate for PHAs synthesis. It contains mainly lactose (44–46%), proteins (6–8%), and lesser amounts of lactic acid (0.5–6.4%), fats (0.06–0.8%), riboflavin (vitamin B2), mineral salts such as NaCl and KCl (0.46–10%), calcium (1.2–1.6%), and phosphate (2.0–4.5%) [46]. There are a number of publications that proved the possibility of using cheese whey (CW) as a carbon source for the growth and PHAs synthesis and accumulation by bacteria. The value of using CW and its derivatives is known because it would decrease the overall costs of PHA production and solve an environmental problem caused by their high organic load.
However, finding bacteria able to produce PHAs when growing on whey is challenging due to the fact that some microorganisms regarded as the best PHA producers were uncapable of directly metabolizing whey towards PHA synthesis. It is known that Cupriavidus necator or Alcaligenes eutrophus are able to accumulate up to 80% of PHAs cultured on glucose, but they are unable to grow and synthesize these biopolyesters using lactose, the predominant carbon source of whey [84]. It was proved that only Alcaligenes latus known as the efficient PHA producer was capable of converting whey lactose into P(3HB) homopolymer resulting in its final concentration of 1.28 g L−1 and the productivity level of 0.11 g L−1 h−1 [85]. Many studies reported that due to complexity of whey, there was a need to conduct its series of pretreatments. Das et al. [46] revealed that ultrafiltered cheese whey better supported the P(3HB) synthesis and accumulation than the whole cheese whey. The authors confirmed that Bacillus megaterium NCIM 5472 was able to accumulate up to 75.5% of P(3HB) homopolymer of its cell dry weight generating a P(3HB) yield of 8.29 g L−1. It is so far the highest reported P(3HB) concentration. The above-mentioned results proved that after ultrafiltration some proteins that reduced the inherent nitrogen concentration in media could be removed and in consequence an increase in the homopolymer concentration could be observed. Furthermore, the attempts were made to reduce the proteins content by acidifying whey to a pH close to 4 [50]. After a heat treatment, centrifugation and filtration, the resulted whey supernatant was used as a carbon source in a culture medium. However, it was observed that the removal of most whey proteins led to a decrease in the growth of Methylobacterium sp. ZP24 lowering the final polymer yield [86]. Furthermore, Thermus thermofilus HB8 efficiently converted whey supernatant into a PHA heteropolymer containing the short chain length 3-hydroxyvalerate (3HV; 38 mol%) and the medium chain length, 3-hydroxyheptanoate (3HHp; 9.89 mol%), 3-hydroxynanoate (3HN; 16.59 mol%), and 3-hydroxyundecanoate (3HU; 35.42 mol%) [50]. After 24 h of cultivation, the authors determined 35.6% of this copolymer with unique composition [50]. Furthermore, promising results were published by Obruca et al. [47] who improved P(3HB) yields about 50-fold in Bacillus megaterium CCM 2037 grown on cheese whey supernatant by supplementing the culture medium with 1% of ethanol at the beginning of the stationary phase of the bacterial growth. Recently, NCIM 5472 strain of Bacillus megaterium was tested towards scl-copolymer production using cheese whey permeate and propionic acid as a co-substrate. It was confirmed that the highest P(3HB-co-3HV) content of 86.2% of cell dry weight (CDW), and its yield of 3.0 g L−1 was achieved in the cultivation supplemented with 0.1% of propionic acid [52].
Moreover, to improve the PHAs productivity by highly PHA-producing bacteria that are not capable of direct lactose utilization, the chemical or enzymatical conversion of whey lactose into glucose and galactose prior to cultivation processes was conducted. Kucer et al. [48] proved that Halomonas halophila was capable of metabolizing whey lactose after the HCl-catalyzed hydrolysis and producing P(3HB) at the concentration of 3.26 g L−1. Moreoever, Pais et al. [53] described that chemically hydrolyzed cheese whey could be efficiently metabolized by Haloferax mediterranei towards P(3HB-co-3HV) production. The conducted batch cultivation confirmed that this bacterium reached an active biomass concentration of 7.54 g L−1 with a copolymer content of 53% of CDW, and a volumetric productivity of 4.04 g L−1 day−1. Some authors succeeded in the PHA production using glucose and galactose originated from enzymatic hydrolysis of cheese whey lactose. Haloferax mediterranei DSM 1411 was reported to be capable to accumulate up to 66% of P(3HB-co-3HV) copolymer [87]. Nevertheless, it should be highlighted that the use of hydrolysis (especially enzymatic hydrolysis) greatly increases the overall costs of a PHA production process. Therefore, there is a need to look for microorganisms that accumulate high levels of these biopolyesters using whole cheese whey as a carbon source. Bustamante et al. [49] revealed that Caulobacter segnis DSM 29236 in the fed-batch fermentation reached up to 9.25 g L−1 of P(3HB) by directly hydrolyzing lactose from whey. So far, it is the highest reported P(3HB) concentration in the growth of pure bacterial culture using whole cheese whey without any pre-treatments processes. Lower content of PHAs (14% of CDW) was synthesized in Pseudomonas aeruginosa MTCC 7925A cells grown on cheese whey. However, in this case, a unique SCL–LCL-PHA co-polymer was produced consisting of 3-hydroxybutyrate (79.2 mol%), 3-hydroxyvalerate (10.5 mol%) and lesser amounts of 3-hydroxyhexadecanoate (1.9 mol%) and 3-hydroxyoctadecanoate (8.4 mol%) [54]. The above-mentioned results suggested that only a few wild-type bacteria are capable of producing PHAs from whey without the costly series of pre-treatments; however, the PHAs content in their cells was reported to be low. Therefore, it is essential to conduct an extensive bioprospecting of microbes from nature to find bioproducers that will be able to directly metabolize whey lactose and generate high PHA yields.

4. Biodiesel Derived Glycerol as a Feedstock for PHA Production

Waste glycerol from biodiesel is generated in a large quantity as a major byproduct from biodiesel manufacturing and can be used in the pharmaceutical or cosmetics industry. However, it is not economically feasible to refine crude glycerol to high purity for commercial applications [88]. Furthermore, disposal of crude glycerol is associated with a fee and creates environmental concerns [89]. Therefore, many studies have been focused on the finding a way to utilize low-value waste glycerol and produce value-added bioproducts. It has been successfully adopted in green technologies for conversion into PHAs (Table 3). The microbial potential to produce PHAs using waste glycerol derived from biodiesel industry was comprehensively reviewed by Zhu et al. [90] who described the research results obtained from the fermentations processes to year 2013. Therefore, below we provide an overview of trends towards PHAs synthesis using waste glycerol based on selected papers from numerous scientific journals published after year 2013. It was demonstrated that P(3HB) homopolymers, the most common polyhydroxyalkanoate in nature, could be efficiently produced by marine bacteria grown on biodiesel-derived glycerol. Promising results have been published by Takahashi et al. [28] who demonstrated that two isolates LAMA 677 and LAMA 685 from the Mid-South Atlantic ridge cultivated on a medium supplemented with residual glycerol were able to accumulate in their cells up to 52.9 and 53.6% of P(3HB), respectively. It was revealed that nutrients limitation stimulated the synthesis of this homopolymer. In the cultivation of Aeromonas sp. AC_03, higher homopolymer content in the bacterial cells was observed under a phosphorus-limiting environment (22% of CDW) compared to nitrogen starvation (5.6% of CDW) [91]. Moreover, the PL26 strain belonging to Bacillus licheniformis species was found to be an efficient (P3HB-co-3HV) producer and was capable of synthesizing and accumulating up to 64.6% [92]. Higher content of this copolymer was reported by Kumar et al. [93] who revealed that Bacillus thuringiensis EGU45 produced up to 74.8% of (P3HB-co-3HV) after 72 h of the cultivation. Furthermore, the authors observed that PHAs were accumulated in the quite consistent content in the bacterial cells over a period of 96 h suggesting that this bacterium could tolerate high levels of crude glycerol that supports efficient copolymer production. It is especially important regarding large scale production, where the flexibility of harvesting time without affecting the biopolymers, productivity can be considered as great assets. Moreoever, the glycerol-rich-phase from a biodiesel plant supported the growth and the efficient synthesis of P(3HB-4HB-3HV) terpolymers by Cupriavidus necator DSM 545 [94]. In addition, PHAMCL with a high percentage of unsaturated monomers were synthesized by Enterobacter sp. ASC3 isolated from soil that was contaminated with spent cooking oil [62]. This bacterium was capable of accumulating 17.5 g L−1 of P(3HO-co-3H5DD) copolyesters. Higher 3H5DD content (>99 mol%) was reported in the PHAMCL extracted from Acinetobacter sp. ASC1 and Bacillus sp. ASC4. The unsaturated side chain of 3H5DD monomers improved the properties of the extracted PHAMCL and extended their potential future applications. It was also proved that Cupriavidus necator IPT027 synthesized up to 71.1% of copolymers containing novel PHA-constituents as building blocks of medium chains [3HTD (5.54–13.10 mol%)] and long chains [11HHD (53.89–69.05 mol%), 15HPD (4.29–6.04 mol%)] [58]. The authors observed that the composition of crude glycerol influenced not only the content of the monomers in the extracted biopolymers but also their thermal properties.
It should be highlighted that the microbial growth rate of microorganisms grown on biodiesel-derived glycerol could be affected by different impurities such as alcohol, organic and inorganic salts, heavy metals, polyol or ash. It was reported that the high concentration of NaCl in crude glycerol could inhibit bacterial growth and also have a significant effect on PHA productivity and the yield of a final bioproduct [95].

5. Molasses as a Carbon Source for PHA Production

Molasses emerges as an efficient carbon source for PHA synthesis and accumulation because of its nutritional composition, rich in sugar, protein, amino acids and microelements. Given the large market of sugar manufacture, it is possible to obtain residual molasses, from the sugar refinement process, at a low cost. Promising results were published by Kulpreecha et al. [64] who demonstrated that the PHA productivity during Bacillus megaterium BA-019 cultivations depended on the type of the conducted cultures. The authors revealed that fed-batch cultures with molasses enhanced cell dry mass concentration and P(3HB) productivity up to 72.6 g L−1 and 1.27 g L−1 h−1, respectively. The same observations were made by Desouky et al. [63] who noticed that also Bacillus flexus AZU-A2 produced higher level of P(3HB) (6.13 g L−1) with its recovery yield of 92.1% of CDW in fed-batch fermentation compared to batch strategy. Furthermore, the ability to synthesize and accumulate P(3HB) homopolymer during the utilization of sugarcane molasses was found in other species, however in much lower concentrations: Cupriavidus necator [66,96] Bacillus subtilis BPP-19 and BPPI-14 [69].
Besides homopolymers, some microorganisms were found to be able to synthesize scl-mcl-PHA copolymers using molasses as the only carbon source. Hassan et al. [70] observed that Clostridium beijerinckii ASU10 (KF372577) has the potential to produce P(3HB-co-3HO) in the amount of 37.93% of CDW. Gas chromatography–mass spectrometry (GC-MS) analysis confirmed that the extracted copolymer consisted of 47.3 mol% of 3HB and 52.7% of 3HO.
The above-mentioned results seem to be promising; however, some types of molasses such as soy molasses, a byproduct of the soybean processing industry, could be not only a rich source of growth promoters but also could contain high concentrations of growth inhibitors. Therefore, as a substrate in bacterial cultures must be diluted several times to reduce the inhibitory effects prior fermentations processes where it is used as a nutrients source [97].

6. Spent Oils as Substrate for PHA Biosynthesis

Various waste oils have been proven to be the effective carbon sources towards PHA biosynthesis. These feedstocks are considered as the alternative carbon sources for the production of bacterial biopolyesters due to their low market prices, continuous availability and global price consistency. The main bottleneck of using spent oils towards PHA production is their possible inconsistency and heterogeneity due to the exposure to high temperatures, food and air for long periods. Furthermore, chemical reactions during a frying process can form toxic compounds that could hamper the growth of PHA producers. Therefore, the evaluation of characteristics and fatty acid composition of frying oils seems to be essential to optimize the PHAs synthesis process.
Cupriavidus necator is usually employed for PHA production on oily substrates because it was observed that this bacterium was able to utilize triacylglycerols efficiently and was used as a lipid-based PHA producer synthesizing high product yields [98]. The highest P(3HB) content of 89.1% with CDW of 55.4 g L−1 was determined in the fed-batch culture of Cupriavidus necator H16 grown on spent coffee grounds oil [76]. The authors proved that in comparison to other types of waste oils, the utilization of coffee oil resulted in the highest biomass as well as P(3HB) productivity. Recently, PHA accumulation under high salinity seems to be a trendy topic. Halophiles are considered as the “coming stars” for industrial biotechnology because as the extremophilic strains provide many benefits such as the resistance to microbial contamination, the reduction of sterility demands the possibility of a continuous mode of cultivation [99]. There are many reports suggesting that halophiles are capable of converting various inexpensive carbon sources into value-added PHAs; however, a few of them proved that these bacteria valorized efficiently lipid substrates. Kumar and Kim [75] confirmed that Paracoccus sp. LL1 has the potential to serve as a single cell factory for bioconversion of waste cooking oil into high value PHAs. The strain produced 1 g L−1 of P(3HB-co-3HV) corresponding to 30.9% of CDW. Recently, for the first time, different Halomonas sp. were investigated towards their potential to be employed for efficient biotechnological conversion of waste frying oil into polyhydroxyalkanoates [73]. Halomonas neptunia and Halomonas hydrothermalis were identified as good PHA producers with the potential to synthesize about 23% of P(3HB). Moreover, the study showed that initial concentration of NaCl was crucial for PHA yields as well as molecular weight of the extracted polymers. The decrease in NaCl concentration not only improved the biopolyester productivity but also resulted in substantial increase in average molecular weight (Mw) of the produced polymer (from 245 kDa at 100 g L−1 NaCl to 381 kDa at 40 g L−1 NaCl), which is beneficial taking into account thermal processing of this material, where high molecular weight is desired. Furthermore, Halomonas hydrothermalis was reported to biosynthesize P(3HB-co-3HV) (68.83% of CDW), when valerate was utilized as a precursor, with the 3HV fraction in the copolymer reached high values of 50.15 mol% [81]. Van Thuoc et al. [31] found that Salinivibrio sp. M318, a moderately halophilic bacterium isolated from fermenting shrimp paste, was able to use a mixture of waste fish oil and glycerol towards PHA synthesis. The results proved that this strain synthesized up to 51.5% of CDW in the fed-batch culture after 78 h of the fermentation proving that it is a promising wild-type bacterium for the production of PHA from aquaculture residues. Moreover, the highest biosynthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] (54.6% of CDW) was reached when 1,4-butanediol was added as a precursor to the culture medium. The comparable content of P(3HB-co-3HV) copolymer (52.4% of CDW) was determined when sodium valerate was supplied. To the best of our knowledge, the highest content of a scl-copolymer was observed in the cultivation of Bacillus thermoamylovorans supplemented with waste cooking oil. Sangkharak et al. [74] revealed that this bacterium was able to produce up to 87.5% of P(3HB-co-3HV)] with 85 mol% of 3HB and 15 mol% of 3HV content. Moreover, the authors proved that the extracted copolymer can be used as a substrate for P(3HB-co-3HV) methyl ester (3-HAME) production, a biofuel. It has been demonstrated that the properties of 3HAME pass ASTM and Thailand’s fuel standards.
Especially, bacteria belonging to Pseudomonas sp. were reported to be capable of utilizing inexpensive oils into PHAMCL. Ruiz et al. [100] conducted pulse fed-batch fermentation using Pseudomonas chlororaphis 555 grown on waste cooking oil. The authors achieved the highest reported biomass (73 g L−1) and PHA volumetric productivity (0.29 g mcl-PHA−1L−1 h−1). Furthermore, they detected 3-hydroxyoctanoic acid and 3-hydroxydecanoic acid monomers followed by 3-hydroxydodecanoic acid monomers as the dominant subunits with lesser amounts of 3-hydroxyhexanoic acid in the synthesized biopolymer. In addition, saponified waste palm oil seemed to be also a suitable substrate for PHAMCL biosynthesis applying fed-batch fermentations of Pseudomonas sp. Gl01. This strain was able to synthesize up to 48% of PHAMCL at the early stage of the culture (at 17 h) with the monomeric composition contained 3-HHx, 3-HDD, and 3-HTD. Furthermore, due to presence of uneven fatty acids in the waste feedstock, the authors reported a high molar fraction of 3HN (up to 63 mol%) what indicated in the waste palm oil used [91].

7. Other Agro-Food Industrial Wastes as Feedstock for PHA Production

There are many reports confirming that other wastes from the food industry could replace the costly sugars and pure fatty acids commonly used as substrates in the fermentations towards PHA production. The grape pomace, a by-product of wine production, was reported to be efficiently converted by bacteria into PHAs. The attention was focused on this type of a feedstock due to its high concentration of fermentable sugars (mainly glucose and fructose) and the fact that it could be used directly without additional, costly saccharification step. So far, only a few papers described the use of grape pomace as a carbon source for the biosynthesis of PHAs. Kovalcik et al. [78] compared the potential of Cupriavidus necator, Halomonas halophila and Halomonas organivorans to produce P(3HB) by using oil and fermentable sugars derived from grape pomace. However, Cupriavidus necator was found to be the best producer that after 29.5 h of the biofermentation was able to produce 8.5 g L−1 of P(3HB) corresponding to 63% of CDW. Moreover, grape pomace sugar extract was converted into PHAMCL by Pseudomonas species. Follonier et al. [101] proved that during a two-step fermentation process Pseudomonas resinovorans hydrolyzed grape pomace reaching the final biomass concentration of 6.1 g L−1 and PHAMCL accumulation at the level of 21.3 g L−1. The same authors demonstrated the scalability of the above approach by conducting a two-step fermentation in 100 L bioreactor achieving PHAMCL with a high degree of unsaturation. They achieved 14.2 g L−1 of CDW containing 41.1% of poly(3-hydroxyoctanoate-co-3-hydroxy-10-undecenoate) with 53 mol% and 47 mol% of saturated and unsaturated monomers, respectively [102].
Moreover, some fruit peels were successfully transformed by microorganisms into valuable polyhydroxyalkanoates. Vijay and Tarika [27] demonstrated that banana peel can be efficiently utilized by Geobacillus stearothermophillus. It has been shown that this soil bacterium was able to synthesize up to 84.63% of P(3HB) at 96 h of the cultivation. The same authors found that Bacillus subtilis accumulated a comparable content of P(3HB) at 24 h of the fermentation (71.8% of CDW). Bacillus thuringiensis was found to be the lesser efficient PHASCL producer. In the cultivation supplemented with mango peel, it reached a cell dry mass of 7.9 g L−1 with 51.3% of P(3HB) [103].
The halophilic bacteria effectively utilized different agro-industrial hydrolysates and effluents. Pleissner et al. [104] extracted 2.6 g L−1 of P(3HB) from the batch cultivation of Halomonas boliviensis supplemented with bakery waste hydrolysate. The same species was able to synthesize the scl-homopolymer in lesser concentration during fed-batch fermentation (2.1 g L−1 of P(3HB)). A marine bacteria, Halomonas sp. SF2003 seems to be a potential P(3HB) bioproducer in a batch culture fed with agro-industrial effluent. Lemechko et al. [29] found that this halophile converted the effluent from fruit industry into P(3HB) achieving the concentration rate of 1.3 g L−1 at 40 h of the culture. Furthermore, the addition of valeric acid enabled the authors to obtain P(3HB-co-3HV) copolymer with controlled proportion of the 3HV monomer. Recently, it was also revealed that rice mill effluent could be efficiently fermented by Acinetobacter junii to P(3HB) in the concentration of 2.4 g L−1 corresponding to 85.7% content of this homopolymer in the bacterial cells [105].
The production of canned pineapples, especially in Asia, reached 28.4 million tons per year generating a vast volume of waste during processing [71]. Therefore, also such wastes were tested towards value-added bioproducts synthesis. Sukruansuwan and Napathorn [79] demonstrated that a maximum biopolymer content of 60% of CDW was reached by Cupriavidus necator when grown on crude aqueous extract originated from canned pineapple production. The authors revealed that nitrogen-limiting conditions influenced positively on PHA concentration. A similar observation was made by Amini et al. [106] who demonstrated the same bacterium produced P(3HB) in higher concentration cultured on beer brewery wastewater under nutrients limitation. Moreover, nitrogen limitation supported the scl-homopolymer synthesis more efficiently in the culture of Burkholderia sacchari on waste paper hydrolase [107].
Coffee is one of the most valuable products worldwide. Dynamic industrialization and population growth contribute to increase coffee production. However, this manufacture generates a lot of coffee wastes and byproducts among which some of them cannot be reused. Only a few studies presented the possibility of the utilization of coffee wastes to biopolymers production. Obruca et al. [81] proved that spent coffee grounds are an efficient substrate for the bacterial growth and P(3HB) synthesis. The authors demonstrated that Cupriavidus necator was able to accumulate up to 89.1% of PHASCL in a fed-batch cultivation. The final biomass concentration reached 55.4 g L−1 corresponding to PHAs productivity of 1.33 g L−1 h−1. Moreover, Kovalcika et al. [80] studied the potential of Halomonas halophila to produce PHAs from fermentable sugars derived from spent coffee grounds (SCGs). This organism was able to process SCG hydrolysates as a carbon source for its growth, however to prevent the growth inhibition, the hydrolysates have been detoxified using sorbent based on styrene-divinylbenzene based resins. The authors successfully reached 0.95 g L−1 of P(3HB) with its content in bacterial cells of 27% of CDW and the molecular weight of about 440–825 kDa.

8. PHA Production by Genetically Modified Bacteria Using Agro-Industrial Wastes

Many efforts have been made to improve PHA biosynthesis by using genetically recombinant strains (Table 4). Due to ease of molecular manipulations, Escherichia coli is becoming the most promising host bacterium for PHAs production. Escherichia coli mutants result in the best PHA production with whey as a carbon source. Furthermore, hydrolysis steps are not necessary because E. coli possesses enzymes for the breakdown of lactose [108]. According to Pais et al. [109] a recombinant Escherichia coli CML3-1 fused to a lactose-inducible promoter, into the chromosome, via transposition mediated mechanism and harboring PHA synthesis genes from Cupriavidus necator was able to produce 7.83 g L−1 of P(3HB) cultured on cheese whey. Moreover, the influence of molasses on the growth and PHA synthesis by recombinant Escherichia coli harboring phaC1 gene of Pseudomonas sp. LDC-5 was investigated. The PHA yield was higher using a substrate derived from sugar refinery mill (75.5% of CDW) as a carbon source compared to sucrose (65.1% of CDW) [110]. Moreover, biodiesel derived glycerol was successfully employed towards the production of poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) copolymer (P3HB-b-P3HP) with varied composition by Escherichia coli HBP01 contained PHA synthase gene of Cupriavidus necator and two parallel synthetic pathways to produce the 3HB and 3HP monomers, respectively.
It was confirmed that Cupriavidus necator could also be an appropriate host for the PHA synthesis. Cupriavidus necator Re2058/pCB113 harboring PHA synthase gene of Rhodococcus aetherivorans I24 and an enoyl coenzyme A (enoyl-CoA) hydratase gene (phaJ) from Pseudomonas aeruginosa was able to facilitate synthesis of P(3HB-co-3HHx) grown on waste frying oil and low quality waste animal fats and tallow. The mutant synthesized up to 60% of the scl-mcl-copolymer with a final HHx concentration of 19 mol%. However, the HHx content decreased continuously over the cultivation time [116]. The same mutant produced up to 74% of P (78 mL% 3HB-co-22 mol% 3HHx) grown on sludge palm oil, a difficult-to-be-used solid byproduct of the palm oil milling industry [116]. The other recombinant Cupriavidus necator PHB¯4 harboring the PHA synthase gene of Aeromonas caviae (PHB¯4/pBBREE32d13) reached in its cells up to 85% of P(3HB-co-3HHx) copolymers cultured on waste cooking oil as the only carbon source [114]. Whey was also successfully employed for PHAs synthesis by recombinant Cupriavidus necator. Povolo et al. [84] has proved that Cupriavidus necator DSM 545 was able to produce P(3HB) after genetic modification made by an insertion of the lac operon within depolymerase gene (phaZ). When using whey permeate the profiles of growth, and polyester synthesis and accumulation were higher (22% of CDW after 48 h growth) than those obtained in lactose as a control.
Obruca et al. [111] engineered Cupriavidus necator H16 by random chemical mutagenesis that significantly enhanced specific activities of enzymes engaged in oxidative stress response. The recombinants were capable of producing P(3HB) more efficiently compared to the wild-type strain reaching 7.6 g L−1 and 4.9 g L−1, respectively. Moreover, UV mutations have been proven to be useful for enhanced PHAs production. De Paula et al. [119] using UV radiation obtained Pandoraea sp. mutants being able to produce up to 53% of P(3HB-co-3HV) copolymer using crude glycerol from biodiesel industry. Furthermore, sequential mutation of Bacillus licheniformis PHAs-007 using UV and N-methyl-N’-nitro-N-nitrosoguanidine (NTG) improved the level of the synthesis and accumulation of the resulted PHA copolymer. The Bacillus licheniformis M2-12 transformant was able to produce about 2.94 times more copolymers in comparison to the PHAs-007 parent strain. The PHA concentration increased up to 16.23 g L−1 when the culture was supplemented with palm oil mill effluent (POME) as the sole carbon source. Furthermore, gas chromatography analysis confirmed that M2-12 mutant produced a P(3HB-co-3HV-co-3HH) copolymer grown on POME whereas the wild type synthesized a copolymer consisting of 3HB and 3HV monomers [74].
Moreover, engineered metabolic pathways have proven to enhance PHA biosynthesis using waste. Borrero-de Acuña et al. [117] knocked out the tctA gene in Pseudomonas putida KT2440, encoding for an enzyme of the tripartite carboxylate transport system, to improve PHAMCL synthesis. The authors observed a nearly two-fold increment in the PHA volumetric productivity. The recombinant P. putida ΔtctA strain synthesized up to 1.91 g L−1 after 72 h cultivation on 20 g L−1 of waste vegetable oil whereas the wild type produced only 1.01 g L−1 of PHAs. Moreover, it was demonstrated that the disruption of the gene responsible for the depolymerization process of PHA (PHA depolymerase, phaZ) in Pseudomonas putida KT2440 improved the biopolymer titer to the level of 47% of CDW when cultured on biodiesel-derived glycerol [59]. In addition, Sharma et al. [118] proved that a phenazine-deficient mutant of Pseudomonas chlororaphis PA23-63 was able to synthesize PHAMCL at the higher level of 24.8% of CDW compared to the wild-type (10.5% of mcl-PHAs) with a biomass concentration of 4.1 g L−1 when grown on waste frying oil. The extracted PHAMCL composition consisted of a mixture of 3HHx, 3HO, 3HD, 3HDD and 3HTD.

9. Challenges and Future Prospects in PHA Production Using Waste Streams

One of the major issue in the commercialization of PHAs is their overall production cost. During the past decades, great progress has been made in PHA-related research regarding its biosynthesis and possibility to convert different industrial wastes into the value-added biopolymers. However, there are still a number of challenges that need to be addressed to achieve efficient production of PHAs from wastes as feedstocks (Figure 2).
Generally, wastes generated from different industrial companies could have a different chemical composition or some impurities and in consequence can be converted in PHAs with varied efficiency, structure and properties. It is a huge challenge taking into account a large scale process of PHA production. Furthermore, bacteria are not able to metabolize wastes in high amounts. Therefore, research is still needed to be done to adjust a suitable pretreatment method of waste feedstock and to optimize operational parameters and nutrients composition during fermentation processes. Moreover, the development of fermentation strategies and the implementation of genetic engineering tools are essential to enhance PHA productivity.
Moreover, only a few bacteria, able to synthesize PHAs, can be considered useful for biotechnological production of these biopolymers on a commercial scale. There is still a growing need to find new bacteria possessing the potential to produce PHAs when grown on industrial waste. They should be evaluated concerning growth rate on waste fluxes, the ability to wastes conversion into PHAs, ease for genetic manipulation or risk of contamination during bioprocesses. Furthermore, it is crucial to understand their metabolic cycles to utilize waste for potential industrial use due to their high stability under unfavourable growth conditions.
By applying metabolic engineering strategies, the PHA efficiency was successfully improved, and it was also demonstrated that the monomer composition of the produced PHAs can be tuned [59]. However, there is a shortage of studies that aim at using recombinants to produce PHAs at a large scale. Some bacterial species like Escherichia coli or Cupriavidus necator seem to be the potential cell factories for PHA synthesis and accumulation. They can be used as the industrial producers due to their available genomic information, ease for genetic manipulation, high PHA content of cell dry weight, fast growth, and high substrate utilization towards PHAs. Moreover, genetic recombination of biosynthetic pathways could enhance the final biopolymers productivity. Progress in molecular biology helped to improve the ability of bacteria to produce PHA at a high concentration. However, a deeper understanding of genetic and metabolic regulation that drive this biopolyesters synthesis is essential to construct a bacterial platform that will be capable of producing defined PHAs structures and predictable material properties which determine their further applications. More data are needed to be generated from studies with a multi-omics-focused design, to provide more understanding of metabolic pathways response to waste feedstocks used in the microbial PHA production processes. Moreover, synthetic biology tools may be applied to accelerate the bacterial cell growth by changing the binary fission to multiple fission achieving cell division into more than two daughter cells at same time and improved PHA production rate [118].
The PHA extraction process from bacterial cells is also a challenging task. It was proved that the price of the PHA separation could reach 30% of the overall biosynthesis costs [119]. In downstream processing, the extraction step using solvents or detergents is still the most widely used for the recovery of PHAs after the fermentation process. First of all, this strategy is expensive considering an industrial scale and harmful to the environment due to additional waste created after biopolymers recovery step. Therefore, green and environment-friendly extraction has been proposed in multiple recent studies as the solution for toxicity of the chemicals employed, their ecological impact and sustainability. The recent developments of ecofriendly solvent extraction and purification processes were comprehensively reviewed by Li and Wilkins [120]. Furthermore, a new autolytic cell disruption system was proposed as a possible method to control and facilitate the release of PHA granules to the extracellular medium. This approach was successfully tested in programmed self-disruptive Pseudomonas putida BXHL strain, derived from P. putida KT2440, which exhibit alterations in outer membrane integrity that induce lysis hypersensitivity [121]. Moreover, the PHA content in bacteria and its molecular weights can be improved by CRISPRi system. This technology opened a new field in the manipulation of the PHA synthesis-related genes [122].
Utilization of different waste streams as feedstocks in the bacterial fermentations towards PHA production will not only reduce the bioprocess costs but also meet the criterium of wastes cycle which is one of the vision of circular economy.

Author Contributions

Conceptualization, J.M.-C.; writing—original draft preparation, P.M., J.M.-C.; writing—review and editing, P.M., J.M.-C.; supervision, J.M.-C.; funding acquisition, J.M.-C. All authors have read and agreed to the published version of the manuscript.

Funding

Paulina Marciniak is a recipient of a scholarship from the Programme Interdisciplinary Doctoral Studies in Bioeconomy (POWR.03.02.00-00-I034/16-00), which is funded by the European Social Funds.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

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Polymers 13 01731 g001 550
Figure 1. Characteristics of an ideal microorganism that could be used for a large scale production of polyhydroxyalkanoates.
Figure 1. Characteristics of an ideal microorganism that could be used for a large scale production of polyhydroxyalkanoates.
Polymers 13 01731 g001
Polymers 13 01731 g002 550
Figure 2. Challenges in PHA production from waste carbon sources.
Figure 2. Challenges in PHA production from waste carbon sources.
Polymers 13 01731 g002
Table
Table 1. Manufacturers producing PHAs from renewable resources.
Table 1. Manufacturers producing PHAs from renewable resources.
ManufacturerCarbon SourceYear References
Bio-on (Italy) renewable sources or agricultural waste 2007[8]
Hydal (Chech)waste cooking oil2012[9]
Full-Cycle Bioplastic (USA)food waste2015[10]
Biocycle (Brazil)sugar cane2000[11]
Danimer Scientific ( USA) canola oil 2007[12]
Tianjin Green Bioscience (China) plants2013[13]
Tianan Biologic Material Co., (China) dextrose derived from corn or cassava delivered from China2004[14]
Table
Table 2. Possible applications of various types of PHAs.
Table 2. Possible applications of various types of PHAs.
Type of PHAsApplicationReferences
PHASCL
Poly(3-hydroxybutyrate)Blood components, tissue engineering, materials for medical devices, drug delivery system, food packaging[19,32,33,34]
Poly(4-hydroxybutyrate)Agricultural nets[35]
Scl-copolymers
Poly(3-hydroxybutyrate-4-hydroxybutyrate)Matrices, microspheres or micro capsules[36]
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Drug carriers, e.g., in cure of chronic and implant osteomyelitis[37]
Poly(3-hydroxybutyrate-cco-3-hydroxyvalerate) with the addition of poly (L-lactic-co-glycolic acid)Microspheres applied in medicine and tissue engineering[38]
Scl-mcl-copolymers
Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)Scaffolds applied in medicine and tissue engineering[39]
Poly(3-hydroxyoctanoate)/poly(3-hydroxybutyrate) blendsNerve tissue engineering[40]
Table
Table 3. Comparison of PHAs production processes by pure bacteria cultures from waste substrate.
Table 3. Comparison of PHAs production processes by pure bacteria cultures from waste substrate.
BacteriaCarbon SourceCultivation ModeBiomass (g L−1)PHA Content (%)Type of PHAsReferences
Dairy industry wastes
Bacillus megaterium NCIM 5472Cheese whey permeateBatch11.075.5P(3HB)[46]
Bacillus megaterium CCM 2037Whey supernatantBatch2.951.0P(3HB)[47]
Halomonas hydrophilaCheese whey chemically hydrolysatedBatch8.538.3P(3HB)[48]
Caulobacter segnis DSM 29236Cheese wheyFed-batch25.037.0P(3HB)[49]
Thermus thermophilus HB8Whey supernatantBatch1.635.6P(3HB)[50]
Burkholderia sacchari LMF 101Cheese whey + glucoseBatch1.55.1P(3HB)[51]
Bacillus megateriumCheese wheyBatch3.686.6P(3HB-co-3HV)[52]
Haloferax mediterraneiCheese wheyBatch7.553.0P(3HB-co-3HV)[53]
Pseudomonas aeruginosa MTCCCheese wheyBatch0.213.2P(3HB-co-3HV-co-3HHD-co-3HOD)[54]
Biodiesel industry derived byproducts
Marine strain LAMA 685Waste glycerolBatch 0.353.6P(3HB)[28]
Pandoraea sp. MA03Waste glycerolBatch 4.349.0P(3HB)[55]
Burkholderia glumare MA13Waste glycerolBatch 3.941.4P(3HB)[56]
Cupriavidus necatorWaste glycerol from biodiesel industryBatch6.872.0P(3HB-co-3HV)[57]
Cupriavidus necator IPT 027Crude glycerolBatch2.471.1P(3HB-co-3HTP-co-15HPD-co-11HHD)[58]
Burkholderia cepacia IPT 4381.967.4P(3HB-co-3HTD-co-11HHD)
Pseudomonas putida KT2440Waste glycerolBatch4.234.5P(3HTD-co-3HO-co-3HD-co-3H5DD-co-3HDD)[59]
Pseudomonas putida NRRL B-14875Waste glycerolFed-batch9.019.5P(3HHx-co-3HO-co-3HD-co-3HDD)[60]
Pseudomonas chlororaphis subsp. aurantiacaWaste glycerolBatch6.717.1P(3HHx-co-3HTD-co-3HO-3HD-co-3HDD)[61]
Enterobacter sp. ASC3Waste glycerolBatch33.147.2P(3HO-co-3H5DD)[62]
Bacillus sp. ASC47.834.4
Pseudomonas sp. ASC210.728.2
Acinetobacter sp. ASC18.525.4
Molasses
Bacillus flexus AZU-A2Sugarcane molassesBatch4.285.1P(3HB)[63]
Sugarcane molassesFed-batch7.184.3
Sugarcane molasses + acetic acidBatch3.842.4
Bacillus megaterium BA-019Cane molassesBatch8.861.6P(3HB)[64]
Fed-batch72.642.1
Bacillus endophyticusSugarcane molassesBatch3.759.5P(3HB)[65]
Cupriavidus necatorVinasse and sugarcane molassesBatch24.050.0P(3HB)[66]
Bacillus sp. BPPI-19Raw sugarcane molassesBatch0.445.9P(3HB)[67]
Pre-treated sugarcane molasses4.544.7
Bacillus subtilis RS1Sugarcane molassesBatch2.441.7P(3HB)[68]
Bacillus subtilisSugarcane molassesBatch1.516.7P(3HB)[69]
Clostridium beijerinckii ASU10Sugarcane molassesBatch0.937.9P(3HB-co-3HO)[70]
Waste oils
Burkholderia thailandensis E264Digestate of chicken manure combined with waste sunflower oilBatch12.660.0P(3HB)[71]
Ralstonia sp. M91Crude fish oilBatch5.352.0P(3HB)[72]
Halomonas neptuniaWaste frying oilBatch2.923.1P(3HB)[73]
Halomonas halophila0.90.4
Salinivibrio sp. M318Waste fish oil + glycerolBatch10.051.7P(3HB)[31]
waste fish oil + glycerol + 1,4-butanediolFed-batch9.854.6P(3HB-co-4HB)
Waste fish oil + glycerol + sodium heptanoateFed-batch11.253.0P(3HB-co-3HV)
Bacillus thermoamylovoransWaste cooking oilBatch4.087.5P(3HB-co-3HV)[74]
Paracoccus sp. LL1Waste cooking oilsBatch3.2430.8P(3HB-co-3HV)[75]
Other agro-food wastes
Cupriavidus necator H16Oil from spent coffee groundsBatch29.490.1P(3HB)[76]
Fed-batch55.489.1
Cupriavidus necator CCGUG 52238Kitchen wasteFed-batch14.484.5P(3HB)[77]
Cupriavidus necatorGrape winery wasteBatch8.376.8P(3HB)[78]
Cupriavidus necatorBanana peelBatchND79.7P(3HB)[27]
Bacillus siamensisND77.6
Staphylococcus aureus JH1ND70.0
Cupriavidus necator A-04Pineapple core hydrolysateBatch6.135.6P(3HB)[79]
Pineapple peel hydrolysate5.312.7
Halomonas sp. SF2003Agro-industrial wastewatersBatch5.733.0P(3HB)[29]
Halomonas halophilaFermentable sugars from spent coffee groundsBatch3.527.0P(3HB)[80]
Burkholderia cepaciaHydrolysate of spent coffee groundsBatch4.954.8P(3HB-co-3HV)[81]
Halogeometricum borinquense strain E3Cassava wasteBatch3.444.7P(3HB-co-3HV)[30]
Pseudomonas citronellolisApple pulp wasteBatch4.030.0P(3HD-co-3HHx-co-3HDD-co-3HTD-co-3HO)[82]
HB: hydroxybutyrate; HV: hydroxyvalerate; HHx: hydroxyhexanoate; HDD: hydroxydodecanoate; HTD: hydroxytetradecanoate; HPD: hydroxypentadecanoate; HO: hydroxyoctanoate; HHD: hydroxyhexadecanoate; HD: hydroxydecanoate; ND: not determined.
Table
Table 4. An overview of PHAs production by genetically modified bacteria grown on waste carbon sources.
Table 4. An overview of PHAs production by genetically modified bacteria grown on waste carbon sources.
BacteriaCarbon SourceCultivation ModeBiomass Concentration (g L−1)PHA Content (%)Type of PHAsReferences
Cupriavidus necator OE1Waste frying oilBatch8.687.9P(3HB)[111]
Escherichia coliSugar cane molassesFed-batch4.075.0P(3HB)[110]
Ralstonia eutropha NCIMB 11599Wheat bran hydrolysateBatch20.062.5P(3HB)[112]
Escherichia coli P8-X8Cheese wheyFed-batch33.138.7P(3HB)[109]
Escherichia coli CML3-128.221.7
Cupriavidus necator mRePTHydrolyzed whey permeateBatch8.030.0P(3HB)[84]
C. necator Re2058 (pCB113)Waste frying oil and waste animal fatBatch3.178.1P(3HB-co-3HHx)[113]
Cupriavidus necator PHB-4Palm oil-based waste cooking oilBatch11.673.0P(3HB-co-3HHx)[114]
Ralstonia eutropha Re2133Coffee waste oilBatch0.969.0P(3HB-co-3HHx)[115]
Cupriavidus necator Re2058/pCB113Sludge palm oilBatch4.566.0P(3HB-co-3HHx)[116]
Sludge palm oil + tween 204.258.0
Bacillus licheniformis M2-12Palm oil mill effluentBatch11.687.6P(3HB-co-3HV)[74]
Pseudomonas putidaWaste vegetable oilBatch5.038.3P(3HHx-co-3HO-co-3HD-co-3HDD)[117]
Pseudomonas chlororaphis PA23-63Biodiesel-derived waste free fatty acidsBatch4.326.1P(3HHx-co-3HO-co-3HD-co-3HDD-co-3HTD)[118]
Pseudomonas chlororaphis PA23-63Waste canola frying oilBatch4.124.8P(3HHx-co-3HO-co-3HD-co-3HDD-co-3HTD[118]
HB: hydroxybutyrate; HV: hydroxyvalerate; HHx: hydroxyhexanoate; HDD: hydroxydodecanoate; HTD: hydroxytetradecanoate; HO: hydroxyoctanoate; HD: hydroxydecanoate; ND: not determined.
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Marciniak, P.; Możejko-Ciesielska, J. What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria? Polymers 2021, 13, 1731. https://0-doi-org.brum.beds.ac.uk/10.3390/polym13111731

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Marciniak P, Możejko-Ciesielska J. What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria? Polymers. 2021; 13(11):1731. https://0-doi-org.brum.beds.ac.uk/10.3390/polym13111731

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Marciniak, Paulina, and Justyna Możejko-Ciesielska. 2021. "What Is New in the Field of Industrial Wastes Conversion into Polyhydroxyalkanoates by Bacteria?" Polymers 13, no. 11: 1731. https://0-doi-org.brum.beds.ac.uk/10.3390/polym13111731

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