Transcriptional Profiling of Aflatoxin B1-Induced Oxidative Stress and Inflammatory Response in Macrophages
Abstract
:1. Introduction
2. Results
2.1. Toxic Effects of AFB1 on Cell Viability
2.2. Effect of AFB1 on ROS Production in RAW264.7 Cells
2.3. Effect of AFB1 on GSH and MDA Contents in RAW264.7 Cells
2.4. Effect of AFB1 Exposure on Gene Expression of Inflammatory Cytokines
2.5. Transcriptomic Analysis of the Effects of AFB1 on RAW264.7 Cells
2.6. KEGG Analysis of the Effect of AFB1 on Oxidative Phosphorylation Pathway
2.7. Relative Signaling Pathways Mediated by AFB1-Induced Oxidative Stress
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. AFB1 Solutions Preparation
5.2. Cell Culture and Treatment
5.3. Cell Viability Assay
5.4. Determination of Intracellular Reactive Oxygen Species
5.5. Determination of Glutathione and Malondialdehyde
5.6. Analysis of Inflammatory Cytokines Expression
5.7. RNA-Seq and Bioinformatics Analysis
5.8. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes ID | Gene Name | Abbreviations | Log2FC | p-Value | Regulation |
---|---|---|---|---|---|
ENSMUSG00000027398 | Interleukin 1 beta | IL-1β | 0.41 | 0.68 | NS |
ENSMUSG00000025746 | Interleukin 6 | IL-6 | 0.00 | 1.00 | NS |
ENSMUSG00000016529 | Interleukin 10 | IL-10 | 0.00 | 1.00 | NS |
ENSMUSG00000058427 | Chemokine ligand 2 | CXCL2 | 1.09 | <0.001 | UP |
ENSMUSG00000024401 | Tumor necrosis factor alpha | TNF-α | 0.70 | <0.001 | UP |
ENSMUSG00000020826 | nitric oxide synthase 2 | NOS2 | −0.05 | 0.72 | NS |
ENSMUSG00000021253 | Transforming growth factor, beta | TGF-β | −0.34 | 0.99 | NS |
ENSMUSG00000019987 | Arginase 1 | ARG1 | −0.32 | 0.78 | NS |
ENSMUSG00000075122 | CD80 antigen | CD80 | 0.30 | <0.001 | UP |
ENSMUSG00000022901 | CD86 antigen | CD86 | −0.94 | 0.02 | DOWN |
ENSMUSG00000008845 | CD163 antigen | CD163 | −0.36 | 0.74 | NS |
ENSMUSG00000034783 | CD206 antigen | CD206 | −0.27 | 0.89 | NS |
KEGG ID | KEGG Term | p-Value | No. of DEGs | DEGs |
---|---|---|---|---|
ko03320 | PPAR signaling pathway | 0.1676 | 5 | ACAA1B, FABP5, PLIN1, UCP1, CPT1C |
ko04150 | mTOR signaling pathway | 0.2307 | 11 | MAP2K2, LAMTOR5, STRADB, ATP6V1G2, FZD2, SLC3A2, SLC7A5, EIF4EBP1 |
ko04064 | NF-κB signaling pathway | 0.5093 | 4 | BCL2A1B, BCL2A1A, RELB, IKBKG |
ko04151 | PI3K-Akt signaling pathway | 0.7190 | 11 | ITGB7, MAP2K2, LPAR1, COL2A1, LAMB3, EPHA2, CDKN1A, COL1A2, EIF4EBP1, IKBKG, OSM |
ko04630 | JAK-STAT signaling pathway | 0.8721 | 4 | SOCS6, IL12RB2, CDKN1A, OSM |
ko04010 | MAPK signaling pathway | 0.9162 | 6 | MAP2K2, GADD45A, FAS, DDIT3, RELB, IKBKG |
ko04668 | TNF signaling pathway | 0.9344 | 4 | CXCL2, FAS, IKBKG, PGAM5 |
Genes | Primer Position | Primer Sequence | Genebank Number |
---|---|---|---|
GAPDH | Forward | AGGTCGGTGTGAACGGATTTG | GU214026.1 |
Reverse | TGTAGACCATGTAGTTGAGGTCA | ||
NOS2 | Forward | GTTCTCAGCCCAACAATACAAGA | AY090567.1 |
Reverse | GTGGACGGGTCGATGTCAC | ||
ARG1 | Forward | CCCGACTTCTGGGACTTCTG | AB047402.1 |
Reverse | AGTAGGTTCCGAAGACTGGGT | ||
TNF-α | Forward | CCCTCACACTCAGATCATCTTCT | NM_013693.3 |
Reverse | GCTACGACGTGGGCTACAG | ||
TGF-β | Forward | CTCCCGTGGCTTCTAGTGC | NM_011577.2 |
Reverse | GCCTTAGTTTGGACAGGATCTG | ||
IL-6 | Forward | TAGTCCTTCCTACCCCAATTTCC | NM_031168.2 |
Reverse | TTGGTCCTTAGCCACTCCTTC | ||
IL-10 | Forward | GCTCTTACTGACTGGCATGAG | NM_010548.2 |
Reverse | CGCAGCTCTAGGAGCATGTG | ||
CXCL2 | Forward | CCAACCACCAGGCTACAGG | NM_008625.2 |
Reverse | GCGTCACACTCAAGCTCTG | ||
CD86 | Forward | TGTTTCCGTGGAGACGCAAG | NM_019388.3 |
Reverse | TTGAGCCTTTGTAAATGGGCA | ||
CD206 | Forward | CTCTGTTCAGCTATTGGACGC | NM_008625.2 |
Reverse | CGGAATTTCTGGGATTCAGCTTC |
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Ma, J.; Liu, Y.; Guo, Y.; Ma, Q.; Ji, C.; Zhao, L. Transcriptional Profiling of Aflatoxin B1-Induced Oxidative Stress and Inflammatory Response in Macrophages. Toxins 2021, 13, 401. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060401
Ma J, Liu Y, Guo Y, Ma Q, Ji C, Zhao L. Transcriptional Profiling of Aflatoxin B1-Induced Oxidative Stress and Inflammatory Response in Macrophages. Toxins. 2021; 13(6):401. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060401
Chicago/Turabian StyleMa, Jinglin, Yanrong Liu, Yongpeng Guo, Qiugang Ma, Cheng Ji, and Lihong Zhao. 2021. "Transcriptional Profiling of Aflatoxin B1-Induced Oxidative Stress and Inflammatory Response in Macrophages" Toxins 13, no. 6: 401. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060401