Serovars, Virulence and Antimicrobial Resistance Genes of Non-Typhoidal Salmonella Strains from Dairy Systems in Mexico
Abstract
:1. Introduction
2. Results
2.1. Prevalence of Salmonella in the Different Samples
2.2. Antimicrobial Susceptibility Testing
2.3. Serological Identification of Salmonella enterica O Serogroups
2.4. Genomic Characteristics
2.4.1. In Silico Serovar Identification
2.4.2. In Silico Detection of Antimicrobial Resistance Genes
2.4.3. Plasmid Profiling
2.4.4. In Silico Identification of Virulence Genes
2.4.5. In Silico Identification of Salmonella Pathogenicity Island
2.5. Phylogenetic Diversity
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. Salmonella Isolation from Bovine Fecal Samples and Environment
4.3. DNA Extraction
4.4. Detection of ipaB Gene for Salmonella Genus
4.5. Antimicrobial Susceptibility Testing
4.6. Serological Identification of Salmonella enterica O Serogroups
4.7. Genome Sequencing and Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate | Serological Typing | Genome Based in Silico Serotyping |
---|---|---|
SETLX55C | B | E |
SESLP86C | C | C |
SEDGO167B | B | E |
SECOA243PC | C | E |
SECOA252B | C | B |
SEDGO294PC | C | C |
SEDGO298PC | B | E |
SEDGO304PC | C | C |
SECOA310PC | C | C |
SEDGO318B | D | G |
SECOA320C | C | G |
SECOA322PC | C | C |
Isolate | Serovar | Antibiotic Resistance Genes |
---|---|---|
SETLX55C | London | qnrB19, sul2, aph(6)-Id, aph(3′)-Id, aac(6′)-Iaa |
SEBC65B | London | aph(6)-Id_1, tet(A)_6, aph(3′)-Id_1, sul2_2, qnrB19_1, aac(6′)-Iaa_1 |
SESLP86C | Newport | aac(6′)-Iaa_1, dfrA1_8, tet(A)_6, floR_2, sul1_15, mph(A)_2, aadA2_1, blaCARB-2_1, qnrA1_1, qacE |
SEAGS147B | Give | aac(6′)-Iaa_1 |
SEDGO167B | Give | aac(6′)-Iaa_1 |
SECOA225PC | Anatum | aac(6′)-Iaa_1 |
SECOA243PC | Anatum | aac(6′)-Iaa_1 |
SECOA246B | Anatum | aac(6′)-Iaa_1 |
SECOA245B | Reading | tet(C)_3, fosA7_1, qnrB19_1, aac(6′)-Iaa_1 |
SECOA252B | Agona | aac(2′)-IIa_1, fosA7_1, aac(6′)-Iaa_1 |
SECOA255PC | Meleagridis | fosA7_1, aac(6′)-Iaa_1 |
SEDGO269B | Meleagridis | aac(6′)-Iaa_1, fosA7_1, aac(2′)-IIa_1 |
SEDGO298PC | Meleagridis | aac(6′)-Iaa_1, fosA7_1 |
SEDGO276B | Havana | aac(6′)-Iaa_1 |
SEDGO294PC | Montevideo | aac(6′)-Iaa_1 |
SEDGO304PC | Montevideo | aac(6′)-Iaa_1 |
SECOA310PC | Montevideo | aac(6′)-Iaa_1, blaZ_62, mph(C)_2, msr(A)_1 |
SECOA322PC | Montevideo | aac(6′)-Iaa_1 |
SEDGO318B | Cubana | aac(6′)-Iaa_1 |
SECOA320C | Cubana | aac(6′)-Iaa_1 |
Plasmid | Serovar |
---|---|
Col(pHAD28) | London, Reading |
IncQ1 | London |
ColpVC | London |
IncR | Newport |
Col440I | Reading |
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Barrera, S.; Vázquez-Flores, S.; Needle, D.; Rodríguez-Medina, N.; Iglesias, D.; Sevigny, J.L.; Gordon, L.M.; Simpson, S.; Thomas, W.K.; Rodulfo, H.; et al. Serovars, Virulence and Antimicrobial Resistance Genes of Non-Typhoidal Salmonella Strains from Dairy Systems in Mexico. Antibiotics 2023, 12, 1662. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics12121662
Barrera S, Vázquez-Flores S, Needle D, Rodríguez-Medina N, Iglesias D, Sevigny JL, Gordon LM, Simpson S, Thomas WK, Rodulfo H, et al. Serovars, Virulence and Antimicrobial Resistance Genes of Non-Typhoidal Salmonella Strains from Dairy Systems in Mexico. Antibiotics. 2023; 12(12):1662. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics12121662
Chicago/Turabian StyleBarrera, Stephany, Sonia Vázquez-Flores, David Needle, Nadia Rodríguez-Medina, Dianella Iglesias, Joseph L. Sevigny, Lawrence M. Gordon, Stephen Simpson, W. Kelley Thomas, Hectorina Rodulfo, and et al. 2023. "Serovars, Virulence and Antimicrobial Resistance Genes of Non-Typhoidal Salmonella Strains from Dairy Systems in Mexico" Antibiotics 12, no. 12: 1662. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics12121662